Ristola M, Repo H
Department of Bacteriology and Immunology, University of Helsinki, Finland.
APMIS. 1989 Jun;97(6):503-12.
We studied the effects of different variables on luminol-enhanced chemiluminescence (CL) of whole blood, induced by N-formyl-methionyl-leucyl-phenylalanine (FMLP), opsonized zymosan particles (OZP), or phosphate buffered saline (PBS). Use of correction factor based on blood neutrophil count and hemoglobin concentration decreased interindividual variation and improved normality of the frequency distributions of CL responses. In FMLP- and PBS-induced CL, day-to-day variation was not significant, but it was significant in OZP-induced CL. Although storage of blood samples for 8 hours decreased CL, the decrease was not evident in a study of initial activation, i.e. CL measured at one minute after application of FMLP. Our results suggest that FMLP-induced initial activation is well suited for epidemiological studies because it is easy to determine and is affected neither by day-to-day variation nor by storage of the blood samples for a few hours.
我们研究了不同变量对由N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（FMLP）、调理酵母聚糖颗粒（OZP）或磷酸盐缓冲盐水（PBS）诱导的全血鲁米诺增强化学发光（CL）的影响。使用基于血液中性粒细胞计数和血红蛋白浓度的校正因子可减少个体间差异，并改善CL反应频率分布的正态性。在FMLP和PBS诱导的CL中，每日变化不显著，但在OZP诱导的CL中显著。虽然血液样本储存8小时会降低CL，但在初始激活研究中，即应用FMLP后一分钟测量的CL，这种降低并不明显。我们的结果表明，FMLP诱导的初始激活非常适合流行病学研究，因为它易于测定，且不受每日变化或血液样本储存数小时的影响。
