Riaz A A, Wan M X, Schäfer T, Dawson P, Menger M D, Jeppsson B, Thorlacius H
Department of Surgery, Malmö University Hospital, Lund University, Malmö, Sweden, Imperial College School of Medicine, Hammersmith Hospital, London, UK.
Br J Surg. 2002 Dec;89(12):1572-80. doi: 10.1046/j.1365-2168.2002.02279.x.
Leucocyte recruitment is a key feature in ischaemia-reperfusion (I/R)-triggered tissue injury. However, the mechanisms underlying leucocyte-endothelium interactions in the large bowel remain elusive because of a previous lack of models to examine the colonic microcirculation. The aim of this study was to develop and validate a novel method for studying reperfusion-induced leucocyte-endothelium interactions in the colon.
The superior mesenteric artery was occluded for 30 min in male C57/Bl6 mice and leucocyte responses were analysed in colonic venules after 30-240 min of reperfusion. Analysis of leucocyte rolling and adhesion in colonic venules was made possible by an inverted approach using intravital fluorescence microscopy.
Thirty minutes of ischaemia and 120 min of reperfusion induced the strongest and most reproducible increase in leucocyte rolling and adhesion. This was associated with a significant increase in colonic levels of malondialdehyde (MDA). Administration of allopurinol and superoxide dismutase reduced I/R-induced leucocyte responses in a dose-dependent manner. Pretreatment with allopurinol attenuated the tissue content MDA in the colon by more than 60 per cent.
A new method for examining I/R-induced leucocyte responses in the colonic microcirculation is described. Oxygen free radicals play an important role in triggering leucocyte rolling and adhesion in colonic venules.
白细胞募集是缺血再灌注（I/R）引发的组织损伤的关键特征。然而，由于此前缺乏用于研究结肠微循环的模型，大肠中白细胞与内皮细胞相互作用的潜在机制仍不清楚。本研究的目的是开发并验证一种用于研究再灌注诱导的结肠白细胞与内皮细胞相互作用的新方法。
在雄性C57/Bl6小鼠中，肠系膜上动脉闭塞30分钟，再灌注30 - 240分钟后分析结肠小静脉中的白细胞反应。通过使用活体荧光显微镜的倒置方法实现对结肠小静脉中白细胞滚动和黏附的分析。
30分钟的缺血和120分钟的再灌注诱导白细胞滚动和黏附出现最强且最可重复的增加。这与结肠中丙二醛（MDA）水平的显著升高相关。给予别嘌呤醇和超氧化物歧化酶以剂量依赖方式降低I/R诱导的白细胞反应。用别嘌呤醇预处理使结肠中的组织MDA含量降低超过60%。
描述了一种用于检查I/R诱导的结肠微循环中白细胞反应的新方法。氧自由基在触发结肠小静脉中的白细胞滚动和黏附方面起重要作用。
