Faust K B, Chiantella V, Vinten-Johansen J, Meredith J H
Department of Surgery, Bowman Gray School of Medicine, Wake Forest University Medical Center, Winston-Salem, North Carolina 27103.
Am Surg. 1988 Dec;54(12):709-19.
Total injury in ischemic skeletal muscle is a function of ischemic damage and reperfusion injury. In an attempt to decrease reperfusion injury, we gave the oxygen-derived free radical scavengers allopurinol, superoxide dismutase, or mannitol during reperfusion of canine gracilis muscle made ischemic for 4 hours. We measured muscle O2 consumption (MVO2), and tissue calcium, water, and adenosine triphosphatase (ATP) before ischemia, after ischemia, and at 5 minutes and 60 minutes of reperfusion. The results at 60 minutes showed no improvement in MVO2 or ATP. In fact, ATP was significantly depressed with allopurinol and superoxide dismutase treatment, and tissue edema did not decrease in any of the groups. We conclude that the simple addition of oxygen-derived free radical scavengers during the initial reperfusion of totally ischemic skeletal muscle does not attenuate reperfusion injury.
缺血性骨骼肌的总损伤是缺血损伤和再灌注损伤共同作用的结果。为了减少再灌注损伤，我们在犬类股薄肌缺血4小时后的再灌注过程中给予了氧自由基清除剂别嘌呤醇、超氧化物歧化酶或甘露醇。我们测量了缺血前、缺血后以及再灌注5分钟和60分钟时的肌肉耗氧量（MVO2）、组织钙、水和三磷酸腺苷酶（ATP）。60分钟时的结果显示MVO2或ATP没有改善。事实上，别嘌呤醇和超氧化物歧化酶处理使ATP显著降低，且所有组的组织水肿均未减轻。我们得出结论，在完全缺血的骨骼肌初始再灌注期间单纯添加氧自由基清除剂并不能减轻再灌注损伤。
