Nakamura Ken-Ichi, Izumiyama-Shimomura Naotaka, Sawabe Motoji, Arai Tomio, Aoyagi Yukitoshi, Fujiwara Mutsunori, Tsuchiya Eiju, Kobayashi Yasuhito, Kato Motonobu, Oshimura Mitsuo, Sasajima Koji, Nakachi Kei, Takubo Kaiyo
Department of Clinical Pathology, Tokyo Metropolitan Institute of Gerontology, Japan.
J Invest Dermatol. 2002 Nov;119(5):1014-9. doi: 10.1046/j.1523-1747.2002.19523.x.
We investigated progressive telomere shortening in normal human epidermis and lingual epithelium during aging, and attempted, in particular, to ascertain whether the telomere shortening that accompanies aging occurs at the same rate in different tissues. We studied telomeric DNA integrity, and estimated annual telomere loss, in 52 specimens of epidermis and 48 specimens of lingual epithelium collected at autopsy from subjects who had died at ages between 0 and 101 y. Most of the DNA samples were measured twice by southern blot hybridization. In addition, the correlation between telomere lengths in the two types of tissues was examined. The telomere reduction rates in epidermis and lingual epithelium were 36 bp and 30 bp per y, respectively, and these were significantly different. The rates obtained by the second measurements in epidermis and lingual epithelium were 39 and 32 bp per y, respectively, and these were also significantly different. The mean telomere lengths in the epidermis of eight neonates and the lingual epithelium of seven neonates were 13.2+/-1.0 and 13.8+/-1.0 kb, respectively. Comparison of telomere lengths in the two tissues for 41 paired samples showed that the mean telomere length in the epidermis (10.7+/-2.3 kb) was less than that in the lingual epithelium (12.4+/-2.5 kb); however, statistical analysis revealed a very significant relationship between epidermal and lingual epithelial telomere length (r=0.842, p<0.0001). These results indicate that the telomeres in epidermis and lingual epithelium are characterized by tissue-specific loss rates.
我们研究了正常人类表皮和舌上皮在衰老过程中的端粒逐渐缩短情况，尤其试图确定衰老过程中伴随的端粒缩短在不同组织中是否以相同速率发生。我们研究了端粒DNA完整性，并估计了从0至101岁死亡受试者尸检采集的52份表皮标本和48份舌上皮标本中的年端粒丢失情况。大多数DNA样本通过Southern印迹杂交进行了两次测量。此外，还检查了两种组织中端粒长度之间的相关性。表皮和舌上皮的端粒减少率分别为每年36 bp和30 bp，且这些差异具有统计学意义。表皮和舌上皮第二次测量得到的速率分别为每年39 bp和32 bp，这些差异同样具有统计学意义。8名新生儿表皮和7名新生儿舌上皮的平均端粒长度分别为13.2±1.0 kb和13.8±1.0 kb。对41对配对样本的两种组织中端粒长度进行比较，结果显示表皮的平均端粒长度（10.7±2.3 kb）小于舌上皮（12.4±2.5 kb）；然而，统计分析显示表皮和舌上皮端粒长度之间存在非常显著的关系（r = 0.842，p < 0.0001）。这些结果表明，表皮和舌上皮中的端粒具有组织特异性的丢失率特征。
