Vyas Yatin M, Maniar Hina, Dupont Bo
Immunology Program, Sloan-Kettering Institute for Cancer Research, New York, NY 10021, USA.
Immunol Rev. 2002 Nov;189:161-78. doi: 10.1034/j.1600-065x.2002.18914.x.
Recent applications of imaging approaches and other methods of cell biology have provided high-resolution visualization of the location of fluorescent proteins in living and fixed cells during cell-cell interactions between lymphocytes, antigen presenting cells and target cells. We review the composition and dynamics of molecular and cytoskeletal events occurring during natural killer cell interactions with susceptible and nonsusceptible target cells. The natural killer cell immune synapse and the concomitant changes in cytoskeletal components and cytoplasmic organelles are described. The findings are compared with the observations made in T helper cells and cytotoxic T cells. It is concluded that the cytolytic immune synapses display spatial-temporal dynamics that are accelerated as compared with T helper cells. In addition, the cytolytic conjugates have unique characteristics relating to their effector function. Furthermore, the natural killer cell immune synapses in cytolytic and noncytolytic interactions are distinctly different and display patterns consistent with characteristic signaling pathways identified in biochemical studies of disrupted cells. The precise relationship between different stages of the natural killer cell immune synapse formation and progression in signal transduction pathways is yet to be established.
成像方法及细胞生物学其他方法的最新应用，已能在淋巴细胞、抗原呈递细胞与靶细胞间的细胞 - 细胞相互作用过程中，对活细胞和固定细胞内荧光蛋白的位置进行高分辨率可视化观察。我们综述了自然杀伤细胞与易感和不易感靶细胞相互作用期间发生的分子及细胞骨架事件的组成和动态变化。描述了自然杀伤细胞免疫突触以及细胞骨架成分和细胞质细胞器随之发生的变化。将这些发现与在辅助性T细胞和细胞毒性T细胞中所做的观察结果进行了比较。得出的结论是，与辅助性T细胞相比，溶细胞免疫突触呈现出加速的时空动态变化。此外，溶细胞共轭物具有与其效应器功能相关的独特特征。而且，溶细胞和非溶细胞相互作用中的自然杀伤细胞免疫突触明显不同，并且呈现出与在破碎细胞的生化研究中确定的特征性信号通路一致的模式。自然杀伤细胞免疫突触形成的不同阶段与信号转导通路进展之间的确切关系尚待确定。
