Lee Seon Hwa, Kim Kyung Mee, Jung Byung Hwa, Chung Woung Youn, Park Cheong Soo, Chung Bong Chul
Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul, 130-650, South Korea.
Cancer Lett. 2003 Jan 10;189(1):27-32. doi: 10.1016/s0304-3835(02)00514-1.
To evaluate the potential effect of estrogens in premenopausal female thyroid cancer, the concentrations of 14 estrogens were quantitatively determined in the urine of pre- and post-operative patients with thyroid papillary cancer (18 patients case, 26 approximately 54 years) and normal female subjects (20 cases, 31 approximately 52 years). The highly sensitive gas chromatography-mass spectrometry-selected ion-monitoring method was used for estrogens analysis. And an estrogen-oxidative metabolism and 16alpha-hydroxyestrone to 2-hydroxyestrone (16alpha-OH E1/2-OH E1) which is the two primary and competing site of estrogen-oxidation, were determined. Catechol estrogens, including 2-OH E1, were also increased without significant changes of the other estrogen metabolites in pre-operative patients with thyroid papillary cancer compared with normal subjects. The lowest mean value of 16alpha-OH E1/2-OH E1 was remarked in pre-operative patients, and it was significantly different from the ratio of post-operative cases. As a result, it is suggested that the increase of 2-hydroxylation in estrogen metabolism may have a significant association with female thyroid cancer.
为评估雌激素对绝经前女性甲状腺癌的潜在影响，采用高灵敏度气相色谱 - 质谱选择离子监测法，对甲状腺乳头状癌患者（18例，年龄约54岁）手术前后及正常女性受试者（20例，年龄约52岁）尿液中的14种雌激素进行定量测定，并测定雌激素氧化代谢及雌激素氧化的两个主要竞争位点——16α - 羟基雌酮与2 - 羟基雌酮（16α - OH E1/2 - OH E1）的比值。与正常受试者相比，甲状腺乳头状癌术前患者中儿茶酚雌激素（包括2 - OH E1）增加，而其他雌激素代谢产物无显著变化。术前患者的16α - OH E1/2 - OH E1比值最低，且与术后病例的比值有显著差异。结果表明，雌激素代谢中2 - 羟化作用的增强可能与女性甲状腺癌有显著关联。
