利用冲击波将核糖体失活蛋白毒素递送至癌细胞。
Delivery of ribosome-inactivating protein toxin into cancer cells with shock waves.
作者信息
Kodama Tetsuya, Doukas Apostolos G, Hamblin Michael R
机构信息
WEL224, Wellman Laboratories of Photomedicine, Massachusetts General Hospital, 55 Fruit Street, Boston, MA 02114, USA.
出版信息
Cancer Lett. 2003 Jan 10;189(1):69-75. doi: 10.1016/s0304-3835(02)00503-7.
Abstract
We report on the use of shock waves delivered by a shock-tube to permeabilize cancer cells and potentiate the cytotoxicity of the type-1 ribosome-inactivating protein, saporin. We studied human colorectal cancer HT29 and ovarian cancer OVCAR-5 cells, and used two different cytotoxicity assays, colony formation and loss of mitochondrial activity. A single shock wave and saporin (10(-9) M) produced significant toxicity not seen with either shock wave or drug alone. Increasing the number of shock waves up to five further increased cytotoxicity. Higher toxicity was seen with the clonogenic assay compared to MTT assay. Shock waves may have applications in promoting cytoplasmic delivery of toxins into cancer cells after intratumoral injection.
摘要
我们报告了使用激波管产生的冲击波使癌细胞通透化，并增强1型核糖体失活蛋白皂草素的细胞毒性。我们研究了人结肠直肠癌HT29细胞和卵巢癌OVCAR-5细胞，并使用了两种不同的细胞毒性检测方法，即集落形成和线粒体活性丧失检测。单个冲击波和皂草素（10^(-9) M）产生了单独使用冲击波或药物时未观察到的显著毒性。将冲击波数量增加至五个进一步增强了细胞毒性。与MTT检测相比，克隆形成检测显示出更高的毒性。冲击波可能在瘤内注射后促进毒素向癌细胞的胞质递送方面具有应用价值。
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