Li Mingmei
Department of Ultrasound Medicine, Xintai People's Hospital.
Afr Health Sci. 2020 Jun;20(2):815-821. doi: 10.4314/ahs.v20i2.35.
Chemotherapy is one of the important medical option for the cancer therapy, but still new combination of therapeutic treatment methods are needed to achieve efficient anti tumor activity. In this work, leaf extract was utilized as a bio reductant for the fabrication of the Gold nanoparticles (Au-NPs) by an ecological approach without using any harmful chemical. This work also evaluates whether the combined effect of chemotherapy along with Au-NPs and Ultrasound (US) is effective over the tumors or not.
About 10 mL of plant extract was added to 1mM of HAuCl4 solution (20.0 mL) and pH was maintained at 12 and allowed for stirring for half an hour. The change in solution color from yellow to purple signified the Au-NPs formation.
XRD results and TEM images confirmed the formation of crystalline Au-NPs with sizes ranging from 23-30 nm. Further, biological studies revealed that the combination of Au-NPs and US along with chemotherapy improved the impact of anti-cancer drug.
In conclusion, leaf extract mediated synthesis of Au-NPs by an eco-friendly approach was reported in this work. The plant biomolecules of the extract were involved in the reduction and capping of the Au-NPs formed. The major conclusion is that addition of Au-NPs with chemotherapy and ultrasound has shown more effective anti-tumor activity.
化疗是癌症治疗的重要医学手段之一，但仍需要新的治疗方法组合以实现高效的抗肿瘤活性。在本研究中，叶提取物被用作生物还原剂，通过生态方法制备金纳米颗粒（Au-NPs），无需使用任何有害化学物质。本研究还评估了化疗联合Au-NPs和超声（US）对肿瘤是否有效。
向1 mM的HAuCl4溶液（20.0 mL）中加入约10 mL植物提取物，将pH值维持在12并搅拌半小时。溶液颜色从黄色变为紫色表明Au-NPs形成。
XRD结果和TEM图像证实形成了尺寸范围为23 - 30 nm的结晶Au-NPs。此外，生物学研究表明，Au-NPs与US联合化疗提高了抗癌药物的疗效。
总之，本研究报道了通过环保方法利用叶提取物介导合成Au-NPs。提取物中的植物生物分子参与了所形成的Au-NPs的还原和包覆。主要结论是，Au-NPs与化疗和超声联合使用显示出更有效的抗肿瘤活性。
