Chung Yoon Hee, Kim Eun Jung, Shin Chung Min, Joo Kyeung Min, Kim Myeung Ju, Woo Haing Won, Cha Choong Ik
Department of Anatomy, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul 110-799, South Korea.
Brain Res. 2002 Nov 29;956(2):312-8. doi: 10.1016/s0006-8993(02)03562-x.
Although the role of cAMP-response-element-binding protein (CREB) binding protein (CBP) in the neuroprotective mechanisms has been the focus of many studies, very little is known about the expression or function of CBP in aged brains. We have therefore examined age-related changes in CBP expression in the cerebral cortex and hippocampus with an immunohistochemical technique. In the cerebral cortex, the distribution patterns were not different between adult and aged groups, but the staining intensity of CBP was significantly decreased in aged rats. In the hippocampus, a distinct immunoreactivity pattern was observed in the CA1-3 areas and dentate gyrus. CBP immunoreactivity was significantly deceased in the pyramidal layer of CA1-3 regions in aged hippocampus. In the dentate gyrus of aged rats, significant decreases were also found in the granule cell layer and polymorphic layer. The first demonstration of age-related decreases in CBP expression in the cerebral cortex and hippocampus may provide useful data for investigating the pathogenesis of age-related neurodegenerative diseases and depression.
尽管环磷酸腺苷反应元件结合蛋白（CREB）结合蛋白（CBP）在神经保护机制中的作用一直是众多研究的焦点，但关于CBP在老年大脑中的表达或功能却知之甚少。因此，我们采用免疫组织化学技术研究了大脑皮质和海马体中CBP表达的年龄相关变化。在大脑皮质中，成年组和老年组的分布模式没有差异，但老年大鼠中CBP的染色强度显著降低。在海马体中，在CA1 - 3区和齿状回观察到明显的免疫反应模式。老年海马体CA1 - 3区锥体层的CBP免疫反应性显著降低。在老年大鼠的齿状回中，颗粒细胞层和多形层也有显著减少。首次证明大脑皮质和海马体中CBP表达随年龄下降，可能为研究年龄相关神经退行性疾病和抑郁症的发病机制提供有用数据。
