Clark M R, Shohet S B
Blood. 1976 Jan;47(1):121-31.
A hybrid erythrocyte model for membrane studies in sickle cell disease has been developed. The model consists of normal red cell membranes containing hemoglobin S and sickle cell membranes containing hemoglobin A. In hybrids, complete hemoglobin exchange has been achieved together with restoration of low membrane permeability to potassium. Normal membranes containing HbS sickle upon deoxygenation and assume the characteristic appearance of irreversibly sickled cells (ISC) after prolonged anoxia. It is suggested that the hybrid model will be useful in defining further the process of ISC formation and in studying the influence of sickle hemoglobin upon the function of the surrounding membrane.
已经开发出一种用于镰状细胞病膜研究的混合红细胞模型。该模型由含有血红蛋白S的正常红细胞膜和含有血红蛋白A的镰状细胞膜组成。在杂交体中，已实现完全的血红蛋白交换，并恢复了对钾的低膜通透性。含有HbS的正常膜在脱氧后会镰变，并在长时间缺氧后呈现不可逆镰状细胞（ISC）的特征性外观。有人认为，该杂交模型将有助于进一步明确ISC的形成过程，并研究镰状血红蛋白对周围膜功能的影响。
