Kirillov D A, Perunova N B, Chelpachenko O E, Elagina N N, Valyshev A V, Bukharin O V
Institute of Cellular and Intracellular Symbiosis of the Urals Branch, Russian Academy of Sciences, Medical Academy, Orenburg, Russia.
Zh Mikrobiol Epidemiol Immunobiol. 2002 Jul-Aug(4):57-9.
The influence of the exometabolites of the fungus S. boulardii, contained in the probiotic preparation "Enterol", on the biological properties of opportunistic and pathogenic enterobacteria of fecal microflora (inactivation of lysozyme, colicin production, hemolytic activity, antibiotic resistance) was studied. The study revealed that the supernatants of S. boulardii decreased antilysozyme activity (ALA) in lactose positive (lac+) and lactose negative (lac-) Escherichia coli and Klebsiella strains, but produced no influence on ALA in Salmonella. In response to the action of S. boulardii exometabolites colicin production in E. coli (lac+) was found to increase, while in E. coli (lac-) colicin production was suppressed. An increase in the sensitivity of lactose negative E. coli to cefazolin and cefotaxime under the action of S. boulardii supenatants was noted. The results obtained in this study show the probable mechanism of the corrective action of "Enterol" on intestinal biocenosis, which should be taken into consideration in the differentiated selection of probiotics for the treatment of intestinal dysbacteriosis.
研究了益生菌制剂“肠乐”中所含的布拉氏酵母菌的胞外代谢产物对粪便微生物群中机会性和致病性肠道细菌生物学特性（溶菌酶失活、大肠杆菌素产生、溶血活性、抗生素耐药性）的影响。研究发现，布拉氏酵母菌的上清液可降低乳糖阳性（lac+）和乳糖阴性（lac-）大肠杆菌及克雷伯菌菌株的抗溶菌酶活性（ALA），但对沙门氏菌的ALA无影响。在布拉氏酵母菌胞外代谢产物的作用下，发现大肠杆菌（lac+）的大肠杆菌素产生增加，而大肠杆菌（lac-）的大肠杆菌素产生受到抑制。注意到在布拉氏酵母菌上清液的作用下，乳糖阴性大肠杆菌对头孢唑林和头孢噻肟的敏感性增加。本研究获得的结果表明了“肠乐”对肠道生物群落的纠正作用的可能机制，在治疗肠道菌群失调的益生菌差异化选择中应考虑这一点。
