Popov N, Pohle W, Rüthrich H L, Schulzeck S, Matthies H
Brain Res. 1976 Jan 16;101(2):283-93. doi: 10.1016/0006-8993(76)90269-9.
Male adult rats were injected intraventricularly with L-[1-3H]fucose. At various intervals, ranging between 30 min and 11 days, one-half of the brain was prepared for microautoradiography, and the hippocampus from the other side was prepared for biochemical investigations. The TCA-precipitable proteins from the hippocampus homogenate were maximally labeled at between 8 and 24 h and remained at a high radioactivity level even 11 days after [3H]-fucose injection, the labeling being predominantly present in the solubilized insoluble proteins. Using gel electrophoretic separation, study of Tris-soluble material indicated a rapid turnover of soluble fucose-containing glycoproteins, whereas several slow-migrating bands of solubulized proteins revealed a time course suggesting the presence of fucose-containing glycoproteins with slower turnover rates. Using microautoradiography, a rapid labeling of neuronal cell bodies of the hippocampus was found, whereas the nuclei were not labeled. Perikarya were maximally labeled 4 h after [3H]fucose application. The radioactive material was continuously transported from the soma into the corresponding fiber layers, the latter being maximally labeled at a pulse interval of one day; even 10 days later a considerable amount of radioactivity could be detected in the neuropil.
将成年雄性大鼠经脑室注射L-[1-³H]岩藻糖。在30分钟至11天的不同时间间隔，取一半大脑用于显微放射自显影，另一侧海马用于生化研究。海马匀浆中三氯乙酸沉淀蛋白在注射[³H]岩藻糖后8至24小时标记程度最高，即使在注射后11天仍保持高放射性水平，标记主要存在于可溶解的不溶性蛋白中。利用凝胶电泳分离，对Tris可溶物质的研究表明含岩藻糖的可溶性糖蛋白周转迅速，而几条迁移缓慢的可溶性蛋白条带显示出一定的时间进程，提示存在周转速率较慢的含岩藻糖糖蛋白。通过显微放射自显影发现，海马神经元胞体迅速被标记，而细胞核未被标记。在应用[³H]岩藻糖后4小时，胞体标记程度最高。放射性物质持续从胞体转运至相应的纤维层，后者在脉冲间隔为一天时标记程度最高；即使在10天后，神经毡中仍可检测到相当量的放射性。
