Popov N, Rüthrich H L, Pohle W, Schulzeck S, Matthies H
Brain Res. 1976 Jan 16;101(2):295-304. doi: 10.1016/0006-8993(76)90270-5.
The incorporation of intraventricularly infected L-[1-3H]fucose into proteins of the hippocampus and visual cortex was studied during the acquisition of a shock-motivated brightness discrimination in rats. The labeling of Tris-soluble proteins from both regions was not significantly changed during learning, whereas solubilized insoluble proteins obtained from the hippocampus revealed an increased fucose incorporation in learned animals. A significant enhanced incorporation into some distinct, slow-moving, carbohydrate-rich protein bands, separated by polyacrylamide gel electrophoresis, was observed in material from CA1 and CA3 sectors as well as from area dentata of the hippocampus formation during acquisition. The corresponding gel bands from the visual cortex exhibited no differences between trained animals and controls. Jointly performed microautoradiography showed an increased fucose incorporation into most areas of the hippocampus in learned animals compared with active and passive controls. The most significant differences were found to occur mainly in substructures consisting of densely packed neuronal cells.
在大鼠获得电击驱动的亮度辨别能力的过程中，研究了脑室内感染的L-[1-³H]岩藻糖掺入海马体和视觉皮层蛋白质的情况。在学习过程中，这两个区域的Tris可溶性蛋白质的标记没有显著变化，而从海马体获得的可溶不溶性蛋白质显示，在已学习的动物中岩藻糖掺入增加。在习得过程中，从海马结构的CA1和CA3区域以及齿状回区域获取的材料中，通过聚丙烯酰胺凝胶电泳分离出的一些不同的、迁移缓慢的、富含碳水化合物的蛋白质条带中，观察到掺入显著增强。来自视觉皮层的相应凝胶条带在训练动物和对照组之间没有差异。联合进行的显微放射自显影显示，与主动和被动对照组相比，已学习动物的海马体大部分区域的岩藻糖掺入增加。发现最显著的差异主要发生在由密集排列的神经元细胞组成的亚结构中。
