Teti Diana, Visalli Maria, McNair Harold
Department of Experimental Pathology and Microbiology, Section of Experimental Pathology, Azienda Policlinico Universitario, Torre Biologica, IV piano, Via Consolare Valeria, 98125 Messina, Italy.
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Dec 5;781(1-2):107-49. doi: 10.1016/s1570-0232(02)00669-4.
The aliphatic polyamines, putrescine, spermidine and spermine, are normal cell constituents that play important roles in cell proliferation and differentiation. The equilibrium between cellular uptake and release and the balanced activities of biosynthetic and catabolic enzymes of polyamines are essential for normal homeostasis in the proliferation and functions of cells and tissues. However, the intracellular polyamine content increases in hyperplastic or neoplastic growth. Although the involvement of polyamines in physiological and pathological cell proliferation and differentiation has been well established, the role they play is quite different in relation to cell systems and animal models and is dependent on inducer agents and stimuli. Also, the experimental procedures used to deplete polyamines have been shown to influence the cell responses. In this paper, the assay methods currently in use for polyamines are reviewed and compared with respect to sensitivity, reproducibility and applicability to routine analysis. The relevance of polyamine metabolism and the uptake/release process in many physiological and pathological processes is highlighted, and the cellular polyamine pathways are discussed in relation to the possible diagnostic and therapeutic significance of these mediators.
脂肪族多胺，如腐胺、亚精胺和精胺，是正常的细胞成分，在细胞增殖和分化中发挥重要作用。细胞摄取与释放之间的平衡以及多胺生物合成和分解代谢酶的平衡活性对于细胞和组织增殖及功能的正常稳态至关重要。然而，在增生性或肿瘤性生长中细胞内多胺含量会增加。尽管多胺参与生理和病理细胞增殖及分化已得到充分证实，但其所起的作用因细胞系统和动物模型而异，并且取决于诱导剂和刺激因素。此外，已表明用于消耗多胺的实验程序会影响细胞反应。本文对目前用于多胺的测定方法进行了综述，并在灵敏度、重现性和常规分析适用性方面进行了比较。强调了多胺代谢以及摄取/释放过程在许多生理和病理过程中的相关性，并讨论了细胞多胺途径与这些介质可能的诊断和治疗意义。
