嗜纤维梭菌纤维小体中负责木聚糖降解的关键亚基XynA的木聚糖酶和乙酰木聚糖酯酶活性。
Xylanase and acetyl xylan esterase activities of XynA, a key subunit of the Clostridium cellulovorans cellulosome for xylan degradation.
作者信息
Kosugi Akihiko, Murashima Koichiro, Doi Roy H
机构信息
Section of Molecular and Cellular Biology, University of California, Davis 95616, USA.
出版信息
Appl Environ Microbiol. 2002 Dec;68(12):6399-402. doi: 10.1128/AEM.68.12.6399-6402.2002.
Abstract
The Clostridium cellulovorans xynA gene encodes the cellulosomal endo-1,4-beta-xylanase XynA, which consists of a family 11 glycoside hydrolase catalytic domain (CD), a dockerin domain, and a NodB domain. The recombinant acetyl xylan esterase (rNodB) encoded by the NodB domain exhibited broad substrate specificity and released acetate not only from acetylated xylan but also from other acetylated substrates. rNodB acted synergistically with the xylanase CD of XynA for hydrolysis of acetylated xylan. Immunological analyses revealed that XynA corresponds to a major xylanase in the cellulosomal fraction. These results indicate that XynA is a key enzymatic subunit for xylan degradation in C. cellulovorans.
摘要
嗜纤维梭菌（Clostridium cellulovorans）的木聚糖酶基因（xynA）编码纤维小体的内切-1,4-β-木聚糖酶XynA，它由一个11家族糖苷水解酶催化结构域（CD）、一个dockerin结构域和一个NodB结构域组成。由NodB结构域编码的重组乙酰木聚糖酯酶（rNodB）表现出广泛的底物特异性，不仅能从乙酰化木聚糖中释放出乙酸盐，还能从其他乙酰化底物中释放乙酸盐。rNodB与XynA的木聚糖酶CD协同作用，用于乙酰化木聚糖的水解。免疫分析表明，XynA对应于纤维小体组分中的一种主要木聚糖酶。这些结果表明，XynA是嗜纤维梭菌中木聚糖降解的关键酶亚基。
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