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1
Xylanase and acetyl xylan esterase activities of XynA, a key subunit of the Clostridium cellulovorans cellulosome for xylan degradation.嗜纤维梭菌纤维小体中负责木聚糖降解的关键亚基XynA的木聚糖酶和乙酰木聚糖酯酶活性。
Appl Environ Microbiol. 2002 Dec;68(12):6399-402. doi: 10.1128/AEM.68.12.6399-6402.2002.
2
The thermostabilizing domain, XynA, of Caldibacillus cellulovorans xylanase is a xylan binding domain.嗜热栖热放线菌木聚糖酶的热稳定结构域XynA是一个木聚糖结合结构域。
Biochem J. 2000 Mar 15;346 Pt 3(Pt 3):583-6.
3
Nucleotide sequences of two contiguous and highly homologous xylanase genes xynA and xynB and characterization of XynA from Clostridium thermocellum.来自热纤梭菌的两个相邻且高度同源的木聚糖酶基因xynA和xynB的核苷酸序列以及XynA的特性分析
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4
An acetylxylan esterase and a xylanase expressed from genes cloned from the ruminal fungus Neocallimastix patriciarum act synergistically to degrade acetylated xylans.从瘤胃真菌梨形新丽鞭毛虫克隆的基因所表达的一种乙酰木聚糖酯酶和一种木聚糖酶协同作用以降解乙酰化木聚糖。
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Homologous xylanases from Clostridium thermocellum: evidence for bi-functional activity, synergism between xylanase catalytic modules and the presence of xylan-binding domains in enzyme complexes.来自热纤梭菌的同源木聚糖酶：双功能活性、木聚糖酶催化模块之间的协同作用以及酶复合物中木聚糖结合结构域存在的证据。
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6
Synergistic effects of cellulosomal xylanase and cellulases from Clostridium cellulovorans on plant cell wall degradation.食纤维梭菌的纤维小体木聚糖酶和纤维素酶对植物细胞壁降解的协同作用。
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7
A novel thermostable multidomain 1,4-beta-xylanase from 'Caldibacillus cellulovorans' and effect of its xylan-binding domain on enzyme activity.一种来自“嗜热纤维芽孢杆菌”的新型耐热多结构域1,4-β-木聚糖酶及其木聚糖结合结构域对酶活性的影响。
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8
Characterization of xylanolytic enzymes in Clostridium cellulovorans: expression of xylanase activity dependent on growth substrates.嗜纤维梭菌中木聚糖分解酶的特性：木聚糖酶活性的表达取决于生长底物。
J Bacteriol. 2001 Dec;183(24):7037-43. doi: 10.1128/JB.183.24.7037-7043.2001.
9
Xylan degradation by the thermophile Clostridium stercorarium: cloning and expression of xylanase, beta-D-xylosidase, and alpha-L-arabinofuranosidase genes in Escherichia coli.嗜热菌粪堆梭菌对木聚糖的降解：木聚糖酶、β-D-木糖苷酶和α-L-阿拉伯呋喃糖苷酶基因在大肠杆菌中的克隆与表达
Biochem Biophys Res Commun. 1990 Jul 16;170(1):368-74. doi: 10.1016/0006-291x(90)91283-x.
10
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3
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3 Biotech. 2011 Oct;1(3):151-159. doi: 10.1007/s13205-011-0019-3. Epub 2011 Aug 19.

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Isolation and Characterization of an Anaerobic, Cellulolytic Bacterium, Clostridium cellulovorans sp. nov.一株厌氧产纤维素菌——纤维梭菌的分离与鉴定
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The Clostridium cellulovorans cellulosome: an enzyme complex with plant cell wall degrading activity.嗜纤维梭菌纤维小体：一种具有植物细胞壁降解活性的酶复合物。
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Nucleotide sequences of two contiguous and highly homologous xylanase genes xynA and xynB and characterization of XynA from Clostridium thermocellum.来自热纤梭菌的两个相邻且高度同源的木聚糖酶基因xynA和xynB的核苷酸序列以及XynA的特性分析
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嗜纤维梭菌纤维小体中负责木聚糖降解的关键亚基XynA的木聚糖酶和乙酰木聚糖酯酶活性。

Xylanase and acetyl xylan esterase activities of XynA, a key subunit of the Clostridium cellulovorans cellulosome for xylan degradation.

作者信息

Kosugi Akihiko, Murashima Koichiro, Doi Roy H

机构信息

Section of Molecular and Cellular Biology, University of California, Davis 95616, USA.

出版信息

Appl Environ Microbiol. 2002 Dec;68(12):6399-402. doi: 10.1128/AEM.68.12.6399-6402.2002.

DOI:10.1128/AEM.68.12.6399-6402.2002
PMID:12450866
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC134393/
Abstract

The Clostridium cellulovorans xynA gene encodes the cellulosomal endo-1,4-beta-xylanase XynA, which consists of a family 11 glycoside hydrolase catalytic domain (CD), a dockerin domain, and a NodB domain. The recombinant acetyl xylan esterase (rNodB) encoded by the NodB domain exhibited broad substrate specificity and released acetate not only from acetylated xylan but also from other acetylated substrates. rNodB acted synergistically with the xylanase CD of XynA for hydrolysis of acetylated xylan. Immunological analyses revealed that XynA corresponds to a major xylanase in the cellulosomal fraction. These results indicate that XynA is a key enzymatic subunit for xylan degradation in C. cellulovorans.

摘要

嗜纤维梭菌（Clostridium cellulovorans）的木聚糖酶基因（xynA）编码纤维小体的内切-1,4-β-木聚糖酶XynA，它由一个11家族糖苷水解酶催化结构域（CD）、一个dockerin结构域和一个NodB结构域组成。由NodB结构域编码的重组乙酰木聚糖酯酶（rNodB）表现出广泛的底物特异性，不仅能从乙酰化木聚糖中释放出乙酸盐，还能从其他乙酰化底物中释放乙酸盐。rNodB与XynA的木聚糖酶CD协同作用，用于乙酰化木聚糖的水解。免疫分析表明，XynA对应于纤维小体组分中的一种主要木聚糖酶。这些结果表明，XynA是嗜纤维梭菌中木聚糖降解的关键酶亚基。