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嗜热栖热放线菌木聚糖酶的热稳定结构域XynA是一个木聚糖结合结构域。

The thermostabilizing domain, XynA, of Caldibacillus cellulovorans xylanase is a xylan binding domain.

作者信息

Sunna A, Gibbs M D, Bergquist P L

机构信息

Department of Biological Sciences, Macquarie University, Sydney, NSW 2109, Australia.

出版信息

Biochem J. 2000 Mar 15;346 Pt 3(Pt 3):583-6.

PMID:10698682
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1220888/
Abstract

We show that the N-terminal 'thermostabilizing domain' (TSD) of the xylanase, XynA, from the thermophilic bacterium Caldibacillus cellulovorans also acts as a xylan binding domain. Affinity electrophoresis experiments show that this TSD selectively binds soluble xylan and binds weakly to hydroxyethylcellulose. Based on this, and previously reported evidence, we propose that xylanase-associated TSDs are xylan binding domains.

摘要

我们发现，嗜热纤维芽孢杆菌（Caldibacillus cellulovorans）的木聚糖酶XynA的N端“热稳定结构域”（TSD）也充当木聚糖结合结构域。亲和电泳实验表明，该TSD选择性结合可溶性木聚糖，与羟乙基纤维素的结合较弱。基于此以及先前报道的证据，我们提出与木聚糖酶相关的TSD是木聚糖结合结构域。

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