环境病原体溃疡分枝杆菌在低温下于两栖动物细胞中生长。
The environmental pathogen Mycobacterium ulcerans grows in amphibian cells at low temperatures.
作者信息
Drancourt Michel, Jarlier Vincent, Raoult Didier
机构信息
Unité des Rickettsies CNRS UPRES A 6020, Faculté de Médecine, IFR 48, Université de la Méditerranée, 13385 Marseille, France.
出版信息
Appl Environ Microbiol. 2002 Dec;68(12):6403-4. doi: 10.1128/AEM.68.12.6403-6404.2002.
Abstract
Mycobacterium ulcerans, the etiological agent of Buruli ulcers, is an environmental pathogen. We cultivated it in an amphibian (XTC-2) cell line that grows at 28 degrees C. By counting of Ziehl-Neelsen-stained mycobacteria and by quantitative PCR analysis, we found that M. ulcerans multiplies rapidly in association with XTC-2 cells. Transmission electron microscopy demonstrated the presence of intracellular M. ulcerans microorganisms. These data suggest an intracellular environmental niche, and we propose use of XTC-2 cells for isolation of M. ulcerans from environmental sources.
摘要
溃疡分枝杆菌是布氏溃疡的病原体，是一种环境病原体。我们在28摄氏度生长的两栖动物（XTC-2）细胞系中培养它。通过对齐尔-尼尔森染色的分枝杆菌进行计数和定量PCR分析，我们发现溃疡分枝杆菌与XTC-2细胞相关时繁殖迅速。透射电子显微镜显示存在细胞内溃疡分枝杆菌微生物。这些数据表明存在细胞内环境生态位，我们建议使用XTC-2细胞从环境来源中分离溃疡分枝杆菌。
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