Ozawa Yoshiyuki, Tanimoto Koichi, Nomura Takahiro, Yoshinaga Masao, Arakawa Yoshichika, Ike Yasuyoshi
Department of Microbiology, Gunma University School of Medicine, Maebashi, Japan.
Appl Environ Microbiol. 2002 Dec;68(12):6457-61. doi: 10.1128/AEM.68.12.6457-6461.2002.
The phenotypes and genotypes of 22 VanA-type vancomycin-resistant enterococci that had been isolated in Japan were examined. The VanA resistance determinant was plasmid mediated in each of the 22 strains. Of the 22 strains, 8 were isolated from different patients and 11 and 3 were obtained from different samples of chickens imported from Thailand and France, respectively. Three of the strains that were isolated from patients and the 11 strains isolated from the Thai chickens showed high-level vancomycin resistance (MICs, 512 to 1,024 micro g/ml) and low-level teicoplanin resistance (MICs, 0.5 to 4 micro g/ml). Each of these strains had three amino acid substitutions in the N-terminal region of the deduced VanS sequence. L50 was converted to V, E54 was converted to Q, and Q69 was converted to H compared to the vanS gene sequence of Tn1546.
对在日本分离出的22株VanA型耐万古霉素肠球菌的表型和基因型进行了检测。22株菌株中的VanA耐药决定簇均由质粒介导。在这22株菌株中，8株分离自不同患者，11株和3株分别从泰国和法国进口的鸡的不同样本中获得。从患者中分离出的3株菌株以及从泰国鸡中分离出的11株菌株表现出高水平的万古霉素耐药性（MICs，512至1024μg/ml）和低水平的替考拉宁耐药性（MICs，0.5至4μg/ml）。与Tn1546的vanS基因序列相比，这些菌株中的每一株在推导的VanS序列的N端区域都有三个氨基酸替换。L50转换为V，E54转换为Q，Q69转换为H。
