Jung Woo Kyung, Lim Ji Youn, Kwon Nam Hoon, Kim Jun Man, Hong Soon Keun, Koo Hye Cheong, Kim So Hyun, Park Yong Ho
Department of Microbiology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul, 151-742, Republic of Korea.
Int J Food Microbiol. 2007 Jan 1;113(1):102-7. doi: 10.1016/j.ijfoodmicro.2006.07.023. Epub 2006 Sep 27.
Enterococci for which the minimum inhibitory concentration (MIC) of vancomycin was >/=8 mg/l were isolated from meat, feces, and raw milk samples collected in Korea from March to November 2003. Among the 243 vancomycin-resistant enterococci (VRE) that were identified the vanA vancomycin resistance gene was carried by 51 Enterococcus faecium and one Enterococcus sp., vanC1 was carried by 151 Enterococcus gallinarum, vanC2 was carried by 39 Enterococcus casseliflavus, and one Enterococcus sp. carried no van genes. Of the isolated enterococci carrying vanA, 4% were found to be highly resistant to gentamicin and 11% were resistant to ampicillin. Further genotyping of the E. faecium isolates carrying vanA using pulsed-field gel electrophoresis (PFGE) revealed extensive heterogeneity. The vancomycin resistance transferability test revealed that only two of the 52 enterococci carrying the vanA gene were able to transfer vancomycin resistance to other enterococci. The VRE were recovered from various animal sources with a particularly high prevalence of E. faecium carrying the vanA gene being found in poultry meat.
2003年3月至11月在韩国采集的肉类、粪便和生乳样本中分离出了万古霉素最低抑菌浓度（MIC）≥8 mg/l的肠球菌。在鉴定出的243株耐万古霉素肠球菌（VRE）中，51株粪肠球菌和1株肠球菌属携带vanA万古霉素耐药基因，151株鹑鸡肠球菌携带vanC1，39株格氏肠球菌携带vanC2，1株肠球菌属未携带van基因。在分离出的携带vanA的肠球菌中，4%对庆大霉素高度耐药，11%对氨苄西林耐药。使用脉冲场凝胶电泳（PFGE）对携带vanA的粪肠球菌分离株进行进一步基因分型，结果显示存在广泛的异质性。万古霉素耐药转移性试验表明，在携带vanA基因的52株肠球菌中，只有2株能够将万古霉素耐药性转移至其他肠球菌。VRE从各种动物源中分离得到，在家禽肉中发现携带vanA基因的粪肠球菌的患病率特别高。
