鼻咽癌相关基因NAG7对鼻咽癌细胞细胞周期和凋亡的影响。

Effects of NPC-associated gene NAG7 on cell cycle and apoptosis in nasopharyngeal carcinoma cells.

作者信息

Tan Chen, Peng Cong, Huang Yu-chen, Zhang Qiu-hong, Tang Ke, Li Xiao-ling, Li Gui-yuan

机构信息

Cancer Research Institute, School of Xiangya Medicine, Central South University, Changsha 410078, P. R. China.

出版信息

Ai Zheng. 2002 May;21(5):449-55.

PMID:12452030

Abstract

BACKGROUND & OBJECTIVE: NPC-associated gene NAG7 was a novel candidate tumor suppressor gene associated with nasopharyngeal carcinoma cloned in our laboratory. This study was designed to investigate the potential effect of NAG7 on the cell cycle and apoptosis of nasopharyngeal carcinoma cell line HNE1 and its molecular mechanism.

METHODS

NAG7 gene was introduced into HNE1 cells using lipofectin transfection technique. The expression level of NAG7 gene was analyzed by Northern blot. Cell cycle, cyclins, and cell apoptosis were detected by flow cytometry, and the expressions of cyclin D1 and cyclin E were detected by Western blot.

RESULTS

NAG7 gene was re-expressed in NAG7 transfected HNE1 cells. Compared with HNE1 cells and vector transfected HNE1 cells, NAG7 transfected HNE1 cells arrested in G0/G1 phase increased (P < 0.05) and cells in S phase decreased (P < 0.05), the apoptosis cells increased (P < 0.05), and the levels of cyclins of A, B1, D1, and E decreased. Furthermore, the expression of cyclin D1 and E decreased in NAG7 transfected HNE1 cells.

CONCLUSION

NAG7 gene re-expression could inhibit overproliferation of NPC cell by delaying the progression of G1 into S in cell cycle and inducing cell apoptosis.

摘要

背景与目的

NPC相关基因NAG7是本实验室克隆的与鼻咽癌相关的新型候选抑癌基因。本研究旨在探讨NAG7对鼻咽癌细胞系HNE1细胞周期及凋亡的潜在影响及其分子机制。

方法

采用脂质体转染技术将NAG7基因导入HNE1细胞。用Northern印迹法分析NAG7基因的表达水平。用流式细胞术检测细胞周期、细胞周期蛋白及细胞凋亡情况，并用Western印迹法检测细胞周期蛋白D1和细胞周期蛋白E的表达。

结果

NAG7基因在转染NAG7的HNE1细胞中重新表达。与HNE1细胞及转染载体的HNE1细胞相比，转染NAG7的HNE1细胞停滞于G0/G1期的细胞增多（P<0.05），S期细胞减少（P<0.05），凋亡细胞增多（P<0.05），细胞周期蛋白A、B1、D1和E的水平降低。此外，转染NAG7的HNE1细胞中细胞周期蛋白D1和E的表达降低。