Brandhorst T Tristan, Rooney Peggy J, Sullivan Thomas D, Klein Bruce
Departments of Pediatrics, University of Wisconsin Medical School, Madison, Wisconsin 53792, USA.
Int J Med Microbiol. 2002 Oct;292(5-6):363-71. doi: 10.1078/1438-4221-00219.
Fungal pathogens have emerged as a public health menace owing to the expanding population of vulnerable patients and to a heightened exposure to fungi in our environment, particularly for the systemic dimorphic fungi that inhabit soil worldwide. A better understanding of these microbes and their pathogenic mechanisms is badly needed to further research into therapeutic options. Advances in the molecular tools for genetic manipulation of Blastomyces dermatitidis have enhanced our ability to study this poorly understood dimorphic fungal pathogen. Recent refinements in gene-transfer technique, new selection markers, reliable reporter fusions and successes in gene targeting have shed light upon the importance of the mycelium-to-yeast transition and the crucial and complex role the BAD1 adhesin plays in pathogenesis.
由于易感患者群体不断扩大以及我们在环境中接触真菌的机会增加，真菌病原体已成为一种公共卫生威胁，特别是对于全球土壤中存在的系统性二态真菌而言。迫切需要更好地了解这些微生物及其致病机制，以进一步研究治疗方案。用于皮炎芽生菌基因操作的分子工具的进展增强了我们研究这种了解甚少的二态真菌病原体的能力。基因转移技术的最新改进、新的选择标记、可靠的报告基因融合以及基因靶向的成功，揭示了菌丝体向酵母转变的重要性以及BAD1粘附素在发病机制中所起的关键而复杂的作用。
