Elguezabal Natalia, Lopitz-Otsoa Fernando, Laín Ana, de Larrinoa Iñigo Fernández, Moragues María Dolores, Pontón José
Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina y Odontología, Universidad del País Vasco, Bilbao, Vizcaya, Spain.
Mycopathologia. 2005 Sep;160(2):97-109. doi: 10.1007/s11046-005-0144-9.
The early diagnosis of mycoses is important for the institution of an effective antifungal therapy. Detection of antibodies against crude antigenic extracts is one of the standard techniques for the diagnosis of most mycoses. However, while these crude antigenic extracts are relatively easy to obtain, they usually show low reproducibility and are not very specific, since antibodies from patients with different mycoses may show cross-reactivity. The application of molecular biology techniques to the study of fungal antigens has allowed the production of recombinant antigens that may help to solve these problems. The purpose of this review is to discuss the use of recombinant fungal antigens in the diagnosis of mycoses.
真菌病的早期诊断对于开展有效的抗真菌治疗至关重要。检测针对粗制抗原提取物的抗体是诊断大多数真菌病的标准技术之一。然而，虽然这些粗制抗原提取物相对容易获得，但它们通常重现性较低且特异性不强，因为不同真菌病患者的抗体可能会出现交叉反应。将分子生物学技术应用于真菌抗原研究，使得重组抗原的产生成为可能，这或许有助于解决这些问题。本综述的目的是讨论重组真菌抗原在真菌病诊断中的应用。
