Gürer Mehmet Ali, Erel Arzu, Erbaş Deniz, Cağlar Kayhan, Atahan Ciğdem
Department of Dermatology, Faculty of Medicine, Gazi University, Besevler, Ankara, Turkey.
Int J Dermatol. 2002 Nov;41(11):768-70. doi: 10.1046/j.1365-4362.2002.01452.x.
Acne rosacea is a dermatosis with unknown etiology. Some studies have reported a high prevalence of Helicobacter pylori infection in acne rosacea. Other studies have reported a decrease in the severity of the lesions of acne rosacea after eradication of H. pylori. H. pylori is a Gram-negative bacterium which colonizes the gastric mucosa and increases the synthesis of oxygen radicals, such as superoxide and proinflammatory cytokines. These cytokines have been demonstrated to stimulate the synthesis of the inflammatory species nitric oxide (NO). In this study, we examined the role of NO in the possible effect of H. pylori in acne rosacea.
Thirty-three acne rosacea patients were included in the study and the control group comprised 20 healthy individuals. The levels of immunoglobulin G antibodies against H. pylori in the serum samples were measured using the enzyme-linked immunoabsorbent assay method. Measurement of nitrate was performed using chemiluminescence in accordance with the method described by Braman and Hendrix (Braman RS, Hendrix SA. Nanogram nitrite and nitrate determination in environmental and biological materials by vanadium(iii) reduction with chemiluminescence detection. Anal Chem 1989; 61: 2715-2718). For statistical analysis, the t-test was used.
The seropositivity of H. pylori in acne rosacea patients was found to be high; however, the serum nitrate levels were found to be normal.
The results of the study indicate that the inflammatory species NO, which has been hypothesized to be associated with H. pylori, has no role in the inflammatory mechanism of acne rosacea.
玫瑰痤疮是一种病因不明的皮肤病。一些研究报告称玫瑰痤疮患者中幽门螺杆菌感染率很高。其他研究则报告了根除幽门螺杆菌后玫瑰痤疮皮损严重程度有所降低。幽门螺杆菌是一种革兰氏阴性菌，它定殖于胃黏膜并增加超氧化物等氧自由基和促炎细胞因子的合成。这些细胞因子已被证明可刺激炎症物质一氧化氮（NO）的合成。在本研究中，我们研究了NO在幽门螺杆菌对玫瑰痤疮可能产生的影响中的作用。
本研究纳入了33例玫瑰痤疮患者，对照组包括20名健康个体。采用酶联免疫吸附测定法检测血清样本中抗幽门螺杆菌免疫球蛋白G抗体的水平。根据Braman和Hendrix描述的方法（Braman RS, Hendrix SA. 通过钒（III）还原和化学发光检测法测定环境和生物材料中的纳克级亚硝酸盐和硝酸盐。分析化学1989；61：2715 - 2718），使用化学发光法测定硝酸盐。进行统计分析时采用t检验。
发现玫瑰痤疮患者中幽门螺杆菌血清阳性率很高；然而，血清硝酸盐水平正常。
研究结果表明，据推测与幽门螺杆菌相关的炎症物质NO在玫瑰痤疮的炎症机制中不起作用。
