Drobniewski Francis, Balabanova Yanina, Ruddy Michael, Weldon Laura, Jeltkova Katya, Brown Timothy, Malomanova Nadezdna, Elizarova Elvira, Melentyey Alexander, Mutovkin Ebgeny, Zhakharova Svetlana, Fedorin Ivan
Guy's King's amd St Thomas' Medical School, King's College Hospital, London, United Kingdom.
Emerg Infect Dis. 2002 Nov;8(11):1320-6. doi: 10.3201/eid0811.020507.
Consecutive patient cultures (140) of Mycobacteriium tuberculosis were collected from five Russian civilian and prison tuberculosis laboratories and analyzed for rifampin (rpoB) and isoniazid resistance (inhA, katG, ahpC); transmission of Beijing family isolates; and the importance of prison and previous therapy in drug resistance. Rifampin, isoniazid, and multidrug resistance occurred in 58.2%, 51.6%, and 44.7% of cultures, respectively; 80% of prison cultures were rifampin resistant. Spoligotyping and variable number tandem repeat (VNTR) fingerprinting divided the isolates into 43 groups. Spoligotyping demonstrated that a high proportion (68.1%) of patients were infected with Beijing family strains and that most (69.1%) were rifampin resistant; the highest proportion (81.6%) occurred in prison. One VNTR subgroup (42435) comprised 68 (72.3%) of the Beijing isolates with a small number of IS6110 types; 50 (73.5%) were rifampin resistant. Rifampin-resistant Beijing isolates are dominant within the patient population, especially among prisoners, and threaten treatment programs.
从俄罗斯五个民用和监狱结核病实验室收集了140份结核分枝杆菌患者连续培养物，分析其对利福平（rpoB）和异烟肼的耐药性（inhA、katG、ahpC）；北京家族菌株的传播情况；以及监狱和既往治疗在耐药性方面的重要性。利福平、异烟肼和多重耐药分别出现在58.2%、51.6%和44.7%的培养物中；80%的监狱培养物对利福平耐药。间隔寡核苷酸分型（Spoligotyping）和可变数目串联重复序列（VNTR）指纹分析将分离株分为43组。Spoligotyping显示，高比例（68.1%）的患者感染了北京家族菌株，且大多数（69.1%）对利福平耐药；最高比例（81.6%）出现在监狱。一个VNTR亚组（42435）包含68株（72.3%）北京分离株，IS6110类型较少；50株（73.5%）对利福平耐药。耐利福平的北京分离株在患者群体中占主导地位，尤其是在囚犯中，对治疗方案构成威胁。
