von Sallmann L, Grimes P
Invest Ophthalmol. 1976 Jan;15(1):27-9.
Prostaglandin E1 (PGE1) suppresses mitotic activity in the epithelial cells of rat lenses maintained in organ culture. At a concentration of 5 x 10(-5)M, PGE1 caused gradual inhibition of mitosis which was first evident after six hours of incubation and was maximum at 50 per cent of control values after 16 and 24 hours in culture. During this period prostaglandin-treated lenses showed no change in the number of cells labeled with H3-thymidine, but the amount of radioactive precursor incorporated per cell was reduced by almost half. At a higher concentration (1 x 10(-4)M) PGE1 evoked a transient mitotic inhibition within the first few hours of culture, but caused cell damage after 24 hours of incubation; a lower dose (1 x 10(-5M) had no influence on mitotic activity.
前列腺素E1（PGE1）可抑制器官培养中大鼠晶状体上皮细胞的有丝分裂活性。在5×10⁻⁵M的浓度下，PGE1引起有丝分裂的逐渐抑制，这在孵育6小时后首次明显，培养16小时和24小时后达到对照值的50%时抑制作用最大。在此期间，经前列腺素处理的晶状体中，用³H-胸腺嘧啶核苷标记的细胞数量没有变化，但每个细胞掺入的放射性前体数量减少了近一半。在较高浓度（1×10⁻⁴M）下，PGE1在培养的最初几个小时内引起短暂的有丝分裂抑制，但孵育24小时后会导致细胞损伤；较低剂量（1×10⁻⁵M）对有丝分裂活性没有影响。
