利用3.4埃反常散射数据和1.9埃天然数据对大肠杆菌乙酸辅酶A转移酶α亚基结构进行自动追踪。
Autotracing of Escherichia coli acetate CoA-transferase alpha-subunit structure using 3.4 A MAD and 1.9 A native data.
作者信息
Korolev S, Koroleva O, Petterson K, Gu M, Collart F, Dementieva I, Joachimiak A
机构信息
Biosciences Division and Structural Biology Center, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439, USA.
出版信息
Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2116-21. doi: 10.1107/s0907444902017055. Epub 2002 Nov 23.
Abstract
The automation of protein structure determination is an essential component for high-throughput structural analysis in protein X-ray crystallography and is a key element in structural genomics. This highly challenging undertaking relies at present on the availability of high-quality native and derivatized protein crystals diffracting to high or moderate resolution, respectively. Obtaining such crystals often requires significant effort. The present study demonstrates that phases obtained at low resolution (>3.0 A) from crystals of SeMet-labeled protein can be successfully used for automated structure determination. The crystal structure of acetate CoA-transferase alpha-subunit was solved using 3.4 A multi-wavelength anomalous dispersion data collected from a crystal containing SeMet-substituted protein and 1.9 A data collected from a native protein crystal.
摘要
蛋白质结构测定的自动化是蛋白质X射线晶体学高通量结构分析的重要组成部分，也是结构基因组学的关键要素。目前，这项极具挑战性的工作依赖于分别能衍射到高分辨率或中等分辨率的高质量天然和衍生化蛋白质晶体的可得性。获得这样的晶体通常需要付出巨大努力。本研究表明，从硒代甲硫氨酸标记的蛋白质晶体中获得的低分辨率（>3.0 Å）相位可成功用于自动结构测定。使用从含有硒代甲硫氨酸取代蛋白质的晶体收集的3.4 Å多波长反常散射数据和从天然蛋白质晶体收集的1.9 Å数据解析了乙酸辅酶A转移酶α亚基的晶体结构。
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