枯草芽孢杆菌ytxM基因产物的结晶及初步X射线分析
Crystallization and preliminary X-ray analysis of the ytxM gene product from Bacillus subtilis.
作者信息
Mehanni M M, Turnbull A P, Sedelnikova S E, Baker P J, Foster S, Rice D W
机构信息
The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, England.
出版信息
Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2138-40. doi: 10.1107/s0907444902015512. Epub 2002 Nov 23.
The ytxM gene product from Bacillus subtilis has been cloned, expressed in Escherichia coli, purified and crystallized by the hanging-drop vapour-diffusion method using PEG 3350 as the precipitant. Multiple-wavelength anomalous dispersive X-ray data have been collected to 2.0 A resolution on a single selenomethionine-incorporated crystal. This crystal belongs to the primitive orthorhombic system, with approximate unit-cell parameters a = 44.3, b = 90.9, c = 136.1 A, alpha = beta = gamma = 90 degrees and two monomers in the asymmetric unit.
来自枯草芽孢杆菌的ytxM基因产物已被克隆，在大肠杆菌中表达，纯化，并通过悬滴气相扩散法以聚乙二醇3350作为沉淀剂进行结晶。在单个掺入硒代甲硫氨酸的晶体上收集了多波长反常色散X射线数据，分辨率达到2.0 Å。该晶体属于原始正交晶系，近似晶胞参数为a = 44.3、b = 90.9、c = 136.1 Å，α = β = γ = 90°，不对称单元中有两个单体。
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