枯草芽孢杆菌2,3-二酮-5-甲基硫代戊基-1-磷酸烯醇酶的结晶及初步X射线分析
Crystallization and preliminary X-ray analysis of 2,3-diketo-5-methylthiopentyl-1-phosphate enolase from Bacillus subtilis.
作者信息
Tamura Haruka, Ashida Hiroki, Koga Shogo, Saito Yohtaro, Yadani Tomonori, Kai Yasushi, Inoue Tsuyoshi, Yokota Akiho, Matsumura Hiroyoshi
机构信息
Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Feb 1;65(Pt 2):147-50. doi: 10.1107/S174430910804311X. Epub 2009 Jan 31.
2,3-Diketo-5-methylthiopentyl-1-phosphate enolase (DK-MTP-1P enolase) from Bacillus subtilis was crystallized using the hanging-drop vapour-diffusion method. Crystals grew using PEG 3350 as the precipitant at 293 K. The crystals diffracted to 2.3 A resolution at 100 K using synchrotron radiation and were found to belong to the monoclinic space group P2(1), with unit-cell parameters a = 79.3, b = 91.5, c = 107.0 A, beta = 90.8 degrees. The asymmetric unit contained four molecules of DK-MTP-1P enolase, with a V(M) value of 2.2 A(3) Da(-1) and a solvent content of 43%.
采用悬滴气相扩散法对枯草芽孢杆菌的2,3-二酮-5-甲基硫代戊基-1-磷酸烯醇酶（DK-MTP-1P烯醇酶）进行了结晶。在293 K下，以聚乙二醇3350作为沉淀剂使晶体生长。利用同步辐射，晶体在100 K下衍射至2.3 Å分辨率，发现其属于单斜空间群P2(1)，晶胞参数a = 79.3、b = 91.5、c = 107.0 Å，β = 90.8°。不对称单元包含四个DK-MTP-1P烯醇酶分子，V(M)值为2.2 ų Da⁻¹，溶剂含量为43%。
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