Wright J L, Churg A
Department of Pathology, University of British Columbia, Vancouver, Canada.
Inhal Toxicol. 2002 Nov;14(11):1153-9. doi: 10.1080/08958370290084836.
To ascertain whether cessation of smoke exposure would decrease small-airway secretory-cell metaplasia, guinea pigs were exposed to daily cigarette smoke using a nose-only exposure system for 4 and 8 mo; an additional group was exposed to smoke for 4 mo and then air recovery for 4 mo ("ex-smokers"). Numbers of secretory cells per millimeter of basement membrane were calculated for each bronchiole from measurements of histologic sections stained with Alcian blue/periodic acid Schiff, and cumulative histograms were constructed. Smoke exposure was associated with a significant increase in numbers of secretory cells at 4 mo and 8 mo, although there was no increase in numbers beyond the 4-mo exposure period. In the ex-smoking group, secretory cells were reduced compared to the 4- or 8-mo smoking group, but continued to be greater than controls. We conclude that, in a guinea pig model, smoking produces secretory-cell metaplasia in the airways and that smoking cessation allows partial recovery of normal structure.
为确定停止接触烟雾是否会减少小气道分泌细胞化生，使用仅经鼻暴露系统让豚鼠每日接触香烟烟雾4个月和8个月；另一组豚鼠接触烟雾4个月，然后空气恢复4个月（“戒烟者”）。通过对用阿尔辛蓝/过碘酸希夫染色的组织学切片进行测量，计算每个细支气管每毫米基底膜的分泌细胞数量，并构建累积直方图。接触烟雾在4个月和8个月时与分泌细胞数量显著增加相关，尽管在4个月接触期后数量没有增加。在戒烟组中，分泌细胞数量比4个月或8个月吸烟组减少，但仍高于对照组。我们得出结论，在豚鼠模型中，吸烟会导致气道分泌细胞化生，戒烟可使正常结构部分恢复。
