Yu Tinghe, Bai Jin, Hu Kai, Wang Zhibiao
Institute of Ultrasound Engineering in Medicine, Chongqing Medical University, China.
Ultrason Sonochem. 2003 Jan;10(1):33-5. doi: 10.1016/s1350-4177(02)00105-0.
Ultrasound could potentiate cytotoxicity of adriamycin on cancer cell line as a result of increased intracellular accumulation ascribed to cavitation. In order to determine which free radical led to increase of drug content, effects of the free radical scavenger and antioxidant on increased intracellular adriamycin accumulation by ultrasound were investigated. The intracellular drug content of adriamycin was lower in the group where histidine was administrated before ultrasound exposure and in the group where mannitol was added after sonication. Drug accumulation was also decreased in groups in which vitamin C administrated either before or after ultrasonic exposure. These results suggested that hydroxyl radical play the leading role in synergism between ultrasound and adriamycin.
由于空化作用导致细胞内阿霉素蓄积增加，超声可增强阿霉素对癌细胞系的细胞毒性。为了确定哪种自由基导致药物含量增加，研究了自由基清除剂和抗氧化剂对超声增加细胞内阿霉素蓄积的影响。在超声暴露前给予组氨酸的组以及超声处理后添加甘露醇的组中，阿霉素的细胞内药物含量较低。在超声暴露前后给予维生素C的组中，药物蓄积也减少。这些结果表明，羟基自由基在超声和阿霉素的协同作用中起主导作用。
