Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Indian J Pharmacol. 2013 Mar-Apr;45(2):126-9. doi: 10.4103/0253-7613.108283.
Cyclophosphamide (CP), a widely used antineoplastic drug causes hemorrhagic cystitis (HC) mainly via induction of oxidative stress. Both vitamin C and histidine have antioxidant properties. The present study aimed to investigate the effects of oral (p.o.) administration of vitamin C and histidine on the CP-induced HC in rats.
The animals were divided into two major groups I and II with four subgroups (a, b, c, and d) in each. Groups I and II were treated with intraperitoneal (i.p.) injections of normal saline and CP (200 mg/kg), respectively, thereafter, normal saline, vitamin C (200 mg/kg), histidine (200 mg/kg) and vitamin C plus histidine were p.o. administered in subgroups a, b, c, and d, respectively, three times (2, 6, and 24 h) after i.p. injections of normal saline and CP. Blood samples were assayed for total antioxidant capacity (TAC) and malondialdehyde (MDA) levels. Histopathological changes of bladder wall were investigated.
The decreased TAC and increased MDA levels of plasma and the severity of hemorrhages, congestion, edema, and leukocyte infiltration of bladder induced by CP were recovered with vitamin C and histidine treatments. Combined treatment with vitamin C and histidine showed a potentiation effect.
The results indicated that vitamin C and histidine attenuated the CP-induced HC by reducing of free radical-induced toxic effects.
环磷酰胺（CP）是一种广泛应用的抗肿瘤药物，主要通过诱导氧化应激引起出血性膀胱炎（HC）。维生素 C 和组氨酸都具有抗氧化特性。本研究旨在探讨口服（p.o.）给予维生素 C 和组氨酸对 CP 诱导的大鼠 HC 的影响。
将动物分为两组，I 组和 II 组，每组又分为四个亚组（a、b、c 和 d）。I 组和 II 组分别接受腹腔（i.p.）注射生理盐水和 CP（200 mg/kg），然后，在 i.p.注射生理盐水和 CP 后 2、6 和 24 小时，分别在亚组 a、b、c 和 d 中 p.o.给予生理盐水、维生素 C（200 mg/kg）、组氨酸（200 mg/kg）和维生素 C 加组氨酸。测定血浆总抗氧化能力（TAC）和丙二醛（MDA）水平。观察膀胱壁的组织病理学变化。
CP 引起的血浆 TAC 降低和 MDA 水平升高，以及膀胱出血、充血、水肿和白细胞浸润的严重程度，经维生素 C 和组氨酸治疗后得到恢复。维生素 C 和组氨酸联合治疗显示出增效作用。
结果表明，维生素 C 和组氨酸通过减少自由基诱导的毒性作用，减轻 CP 诱导的 HC。
