Bagchi D, Garg A, Krohn R L, Bagchi M, Tran M X, Stohs S J
School of Pharmacy, Creighton University, Omaha, NE 68178, USA.
Res Commun Mol Pathol Pharmacol. 1997 Feb;95(2):179-89.
Proanthocyanidins, a group of polyphenolic bioflavonoids, have been reported to exhibit a wide range of biological, pharmacological and chemoprotective properties against oxygen free radicals. We have assessed the concentration-dependent oxygen free radical scavenging abilities of a grape seed proanthocyanidin extract (GSPE), vitamin C and vitamin E succinate (VES) as well as superoxide dismutase, catalase and mannitol against biochemically generated superoxide anion and hydroxyl radical using a chemiluminescence assay and cytochrome c reduction. A concentration-dependent inhibition was demonstrated by GSPE. At a 100 mg/l concentration, GSPE exhibited 78-81% inhibition of superoxide anion and hydroxyl radical. Under similar conditions, vitamin C inhibited these two oxygen free radicals by approximately 12-19%, while VES inhibited the two radicals by 36-44%. The combination of superoxide dismutase and catalase inhibited superoxide anion by approximately 83%, while mannitol resulted in an 87% inhibition of hydroxyl radical. The results demonstrate that GSPE is a more potent scavenger of oxygen free radicals as compared to vitamin C and VES.
原花青素是一类多酚生物类黄酮，据报道，其对氧自由基具有广泛的生物学、药理学及化学保护特性。我们使用化学发光分析法和细胞色素c还原法，评估了葡萄籽原花青素提取物（GSPE）、维生素C、维生素E琥珀酸酯（VES）以及超氧化物歧化酶、过氧化氢酶和甘露醇针对生化产生的超氧阴离子和羟基自由基的浓度依赖性氧自由基清除能力。GSPE表现出浓度依赖性抑制作用。在100 mg/l的浓度下，GSPE对超氧阴离子和羟基自由基的抑制率为78 - 81%。在类似条件下，维生素C对这两种氧自由基的抑制率约为12 - 19%，而VES对这两种自由基的抑制率为36 - 44%。超氧化物歧化酶和过氧化氢酶的组合对超氧阴离子的抑制率约为83%，而甘露醇对羟基自由基的抑制率为87%。结果表明，与维生素C和VES相比，GSPE是一种更强效的氧自由基清除剂。
