Pawellek A, Hewlett G, Rosenbruch M, Kreuter J, Rübsamen-Waigmann H
BHC-PH-R-EU AI-II Virology, Bayer AG, P O Box 101709, 42096, Wuppertal, Germany.
Virus Res. 2002 Dec;90(1-2):365-70. doi: 10.1016/s0168-1702(02)00246-0.
Bovine papillomavirus (BPV) type 2 DNA was inoculated into calf scrotal skin before grafting onto severe combined immunodeficient mice. Inoculation with viral DNA isolated from a bovine wart induced fibropapillomas that exhibited all the morphological features of a BPV infection in cattle. The production of capsid protein and infectious BPV2 particles was demonstrated by immunohistochemistry and a transformed cell focus assay. In contrast, the injection of molecularly cloned viral genomic DNA led to the induction of papilloma-like lesions in the epidermis, but a fibroma was not formed. In addition, only early genes were expressed and infectious virus particles could not be detected. A restriction enzyme accessibility assay suggested that the methylation status of the molecularly cloned BPV2 DNA was different from that of native viral DNA. A possible correlation between methylation status and tumour phenotype is discussed.
在将牛阴囊皮肤移植到严重联合免疫缺陷小鼠之前，将2型牛乳头瘤病毒（BPV）DNA接种到小牛阴囊皮肤中。接种从牛疣中分离的病毒DNA可诱导纤维乳头瘤，其表现出牛BPV感染的所有形态学特征。通过免疫组织化学和转化细胞集落试验证明了衣壳蛋白和传染性BPV2颗粒的产生。相比之下，注射分子克隆的病毒基因组DNA可导致表皮中出现乳头状瘤样病变，但未形成纤维瘤。此外，仅表达早期基因，未检测到传染性病毒颗粒。限制性内切酶可及性分析表明，分子克隆的BPV2 DNA的甲基化状态与天然病毒DNA不同。讨论了甲基化状态与肿瘤表型之间可能的相关性。
