Pawellek A, Hewlett G, Kreuter J, Rübsamen-Waigmann H, Weber O
PH-R Ai2 Virology, Bayer AG, Wuppertal, Germany.
Antimicrob Agents Chemother. 2001 Apr;45(4):1014-21. doi: 10.1128/AAC.45.4.1014-1021.2001.
The report describes the establishment and characterization of a mouse xenograft transplantation model for the study of papillomavirus infection of bovine skin. Calf scrotal skin was inoculated with bovine papillomavirus type 2 before grafting it to the dorsum of severe combined immunodeficient mice. The grafted skin contained epidermis, dermis, and a thin layer of fat. After 5 months the induced warts not only showed histological features of papillomavirus infections but also tested positive for viral DNA and papillomavirus capsid antigen. The formation of infectious virions was demonstrated by inoculation of new transplants with crude extract from the induced warts as well as in a cell culture focus assay. Topical application of bromovinyl-2'-deoxyuridine led to a reduction in viral DNA content in the developing wart. This small-animal xenograft model should be useful for characterizing antiviral compounds and providing an understanding of the regulation of papillomavirus infections.
该报告描述了一种用于研究牛皮肤乳头瘤病毒感染的小鼠异种移植模型的建立及特性。在将小牛阴囊皮肤移植到严重联合免疫缺陷小鼠的背部之前，先接种2型牛乳头瘤病毒。移植的皮肤包含表皮、真皮和一层薄脂肪。5个月后，诱导产生的疣不仅表现出乳头瘤病毒感染的组织学特征，而且病毒DNA和乳头瘤病毒衣壳抗原检测呈阳性。通过用诱导疣的粗提物接种新的移植组织以及细胞培养焦点试验，证实了感染性病毒粒子的形成。局部应用溴乙烯基-2'-脱氧尿苷可使正在形成的疣中的病毒DNA含量降低。这种小动物异种移植模型应有助于对抗病毒化合物进行特性分析，并有助于了解乳头瘤病毒感染的调控机制。
