Lee Young Jin, Hoaglund-Hyzera Cherokee S, Srebalus Barnes Catherine A, Hilderbrand Amy E, Valentine Stephen J, Clemmer David E
Department of Chemistry, Indiana University, Bloomington 47405, USA.
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Dec 25;782(1-2):343-51. doi: 10.1016/s1570-0232(02)00569-x.
The development of a multidimensional approach involving high-performance liquid chromatography (LC), ion mobility spectrometry (IMS) and tandem mass spectrometry is described for the analysis of complex peptide mixtures. In this approach, peptides are separated based on differences in their LC retention times and mobilities (as ions drift through He) prior to being introduced into a quadrupole/octopole/time-of-flight mass spectrometer. The initial LC separation and IMS dispersion of ions is used to label ions for subsequent fragmentation studies that are carried out for mixtures of ions. The approach is demonstrated by examining a mixture of peptides generated from tryptic digestion of 18 commercially available proteins. Current limitations of this initial study and potential advantages of the experimental approach are discussed.
描述了一种用于分析复杂肽混合物的多维方法的开发，该方法涉及高效液相色谱（LC）、离子淌度光谱（IMS）和串联质谱。在这种方法中，肽在被引入四极杆/八极杆/飞行时间质谱仪之前，根据其LC保留时间和淌度（作为离子在氦气中漂移）的差异进行分离。离子的初始LC分离和IMS分散用于标记离子，以便对离子混合物进行后续的碎片研究。通过检测由18种市售蛋白质的胰蛋白酶消化产生的肽混合物来证明该方法。讨论了这项初步研究的当前局限性以及该实验方法的潜在优势。
