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采用离子淌度/飞行时间质谱的偶极共振激发实现碰撞诱导解离。

Implementation of dipolar resonant excitation for collision induced dissociation with ion mobility/time-of-flight MS.

机构信息

Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, 99352, USA.

出版信息

J Am Soc Mass Spectrom. 2014 Apr;25(4):563-71. doi: 10.1007/s13361-013-0815-6. Epub 2014 Jan 28.

DOI:10.1007/s13361-013-0815-6
PMID:24470195
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4113219/
Abstract

An ion mobility/time-of-flight mass spectrometer (IMS/TOF MS) platform that allows for resonant excitation collision induced dissociation (CID) is presented. Highly efficient, mass-resolved fragmentation without additional excitation of product ions was accomplished and over-fragmentation common in beam-type CID experiments was alleviated. A quadrupole ion guide was modified to apply a dipolar AC signal across a pair of rods for resonant excitation. The method was characterized with singly protonated methionine enkephalin and triply protonated peptide angiotensin I, yielding maximum CID efficiencies of 44% and 84%, respectively. The Mathieu q(x,y) parameter was set at 0.707 for these experiments to maximize pseudopotential well depths and CID efficiencies. Resonant excitation CID was compared with beam-type CID for the peptide mixture. The ability to apply resonant waveforms in mobility-resolved windows is demonstrated with a peptide mixture yielding fragmentation over a range of mass-to-charge (m/z) ratios within a single IMS-MS analysis.

摘要

介绍了一种可实现共振激发碰撞诱导解离（CID）的离子淌度/飞行时间质谱（IMS/TOF MS）平台。该平台实现了高效、质量分辨的碎裂，而无需额外激发产物离子，从而缓解了在束型 CID 实验中常见的过度碎裂问题。四极离子阱被修改为在一对棒上施加偶极交流信号以进行共振激发。该方法的特点是使用单质子化的甲硫氨酸脑啡肽和三质子化的肽血管紧张素 I 进行了表征，分别得到了 44%和 84%的最大 CID 效率。对于这些实验，Mathieu q(x,y) 参数设置为 0.707，以最大化赝势阱深度和 CID 效率。将共振激发 CID 与肽混合物的束型 CID 进行了比较。通过对肽混合物施加共振波形，在单个 IMS-MS 分析中，在质荷比（m/z）范围内实现了碎裂。

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