Teplova Marianna, Wilds Christopher J, Wawrzak Zdzislaw, Tereshko Valentina, Du Quan, Carrasco Nicolas, Huang Zhen, Egli Martin
Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235, USA.
Biochimie. 2002 Sep;84(9):849-58. doi: 10.1016/s0300-9084(02)01440-2.
Selenium was incorporated into an oligodeoxynucleotide in the form of 2'-methylseleno-uridine (U(Se)). The X-ray crystal structure of the duplex left open bracket d(GCGTA)U(Se)d(ACGC) right open bracket (2) was determined by the multiwavelength anomalous dispersion (MAD) technique and refined to a resolution of 1.3 A, demonstrating that selenium can selectively substitute oxygen in DNA and that the resulting compounds are chemically stable. Since derivatization at the 2'-alpha-position with selenium does not affect the preference of the sugar for the C3'-endo conformation, this strategy is suitable for incorporating selenium into RNA. The availability of selenium-containing nucleic acids for crystallographic phasing offers an attractive alternative to the commonly used halogenated pyrimidines.
硒以2'-甲基硒代尿苷（U(Se)）的形式掺入到寡脱氧核苷酸中。通过多波长反常色散（MAD）技术确定了双链体[ d(GCGTA)U(Se)d(ACGC) ]（2）的X射线晶体结构，并将其精修至1.3 Å的分辨率，这表明硒可以在DNA中选择性取代氧，且所得化合物化学性质稳定。由于在2'-α位用硒进行衍生化不会影响糖对C3'-内型构象的偏好，该策略适用于将硒掺入RNA中。含硒核酸用于晶体学相位测定为常用的卤代嘧啶提供了一种有吸引力的替代方法。
