Martino V S, López P, Martinez Irujo J J, Sanromán M, Cuevas M T, Santiago E, Lasarte J J, Font M, Coussio J D, Monge A
Instituto de Química y Metabolismo del Fármaco (IQUIMEFA-PROSIVAD) (UBA-CONICET), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113 Buenos Aires, República Argentina.
Phytother Res. 2002 Dec;16(8):778-80. doi: 10.1002/ptr.1065.
Dichloromethane, methanol and aqueous extracts from the leaves of Terminalia triflora were investigated for their inhibitory effect on polymerase and ribonuclease activities of HIV reverse transcriptase.The most potent activity was found in the aqueous extract, which inhibited both polymerase and ribonuclease activities of the enzyme with an IC50 of 1.6 micro g/mL and 1.8 micro g/mL respectively. The antiinfective activity of the extract was demonstrated in HLT4LacZ-IIIB cell culture with an IC50 of 1.0 micro g/mL. The extract was submitted to a purification process by extractive and chromatographic methods. The activity remained in the hydrophillic fraction. Tannins present in this active purified fraction, as determined by TLC and HPLC methods, could account for the anti HIV-RT activity found in the aqueous extract.
对三叶榄仁树叶的二氯甲烷、甲醇和水提取物进行了研究，考察它们对HIV逆转录酶的聚合酶和核糖核酸酶活性的抑制作用。发现水提取物的活性最强，它对该酶的聚合酶和核糖核酸酶活性均有抑制作用，IC50分别为1.6微克/毫升和1.8微克/毫升。在HLT4LacZ-IIIB细胞培养中证明该提取物具有抗感染活性，IC50为1.0微克/毫升。通过萃取和色谱方法对提取物进行了纯化处理。活性保留在亲水部分。通过TLC和HPLC方法测定，该活性纯化部分中存在的单宁可能是水提取物中发现的抗HIV-RT活性的原因。
