Corbel Stéphane Y, Rossi Fabio M V
Biomedical Research Centre, University of British Columbia, 2222 Health Sciences Mall, Vancouver, BC V6T1Z3, Canada.
Curr Opin Biotechnol. 2002 Oct;13(5):448-52. doi: 10.1016/s0958-1669(02)00361-0.
In June this year, the tetracycline-regulated gene expression system (tet system) celebrated its tenth "birthday". In the past ten years a continuous stream of changes made to the tet system's basic components has led to a remarkable improvement in its overall performance. It was not until this year, however, that the full benefits of these improvements became apparent. In particular, usage of the tet system is no longer limited to immortalized cell lines and transgenic animals. In this review, we will describe the obstacles encountered in delivering the tet system's components to primary cells and tissues as well as the methods now used to overcome them. We will also focus on a novel system that is conceptually similar but based on different antibiotic/transcription factor pairs.
今年6月，四环素调控基因表达系统（tet系统）迎来了它的十岁“生日”。在过去十年里，tet系统基本组件不断发生的变化使其整体性能有了显著提升。然而，直到今年，这些改进的全部益处才显现出来。特别是，tet系统的应用不再局限于永生化细胞系和转基因动物。在这篇综述中，我们将描述在将tet系统组件递送至原代细胞和组织过程中遇到的障碍以及目前用于克服这些障碍的方法。我们还将重点介绍一种概念上类似但基于不同抗生素/转录因子对的新型系统。
