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磷脂依赖性(钠 + 钾)-ATP 酶对极性头部基团和酰基侧链的要求

Polar head-group and acyl side-chain requirements for phospholipid-dependent (Na-+ plus K-+)-ATPase.

作者信息

Walker J A, Wheeler K P

出版信息

Biochim Biophys Acta. 1975 Jun 11;394(1):135-44. doi: 10.1016/0005-2736(75)90212-6.

DOI:10.1016/0005-2736(75)90212-6
PMID:124596
Abstract

The abilities of different phospholipids to reactivate a lipid-depleted (Na-+ plus K-+)-ATPase (EC 3.6.1.3) have been compared. The phospholipids contained either the same group of hydrocarbon chains withhh different polar groups, or different hydrocarbon groups wit the same polar group; they were prepared by enzymic modification of the polar group of phosphatidylserine isolated from bovine brain and of phosphatidylcholine extracted from egg. Only the acidic phospholipids reactivated the ATPase but the amount of reactivation depended on the nature of the hydrocarbon chains as well as the polar group. These findings are discussed in relations to the compositions of the two different groups of fatty acyl chains constituting the hydrocarbon portions of the phospholipids.

摘要

比较了不同磷脂使脂质耗竭的(钠 + 钾)-ATP 酶(EC 3.6.1.3)重新激活的能力。这些磷脂要么含有相同的烃链基团但极性基团不同,要么含有不同的烃基团但极性基团相同;它们是通过对从牛脑分离的磷脂酰丝氨酸和从鸡蛋中提取的磷脂酰胆碱的极性基团进行酶促修饰而制备的。只有酸性磷脂能使 ATP 酶重新激活,但重新激活的量取决于烃链的性质以及极性基团。结合构成磷脂烃部分的两组不同脂肪酰链的组成对这些发现进行了讨论。

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