从韩国传统发酵比目鱼中分离出的枯草芽孢杆菌SFF34对胆固醇的降解作用。
Degradation of cholesterol by Bacillus subtilis SFF34 isolated from Korean traditional fermented flatfish.
作者信息
Kim K P, Rhee C H, Park H D
机构信息
Department of Food Science and Technology, Kyungpook National University, Taegu, Korea.
出版信息
Lett Appl Microbiol. 2002;35(6):468-72. doi: 10.1046/j.1472-765x.2002.01219.x.
AIMS
To examine cholesterol degradation by Bacillus subtilis SFF34.
METHODS AND RESULTS
Cholesterol degradation and cholesterol oxidase production by B. subtilis SFF34 were investigated in a medium containing 0.2% cholesterol. In addition, the oxidized product of cholesterol by the purified cholesterol oxidase was detected using a gas chromatograph. Cholesterol oxidase production reached its maximal level (3.14 U ml(-1) after 24 h of incubation in the cholesterol medium. The residual cholesterol content reduced to 0.98 mg g(-1) after 60 h of cultivation in the cholesterol medium. Two cholesterol oxidases were purified from the culture supernatant fluid and their reaction product against cholesterol was identified as 4-cholesten-3-one.
CONCLUSIONS
B. subtilis SFF34 degraded cholesterol and produced a high level of extracellular cholesterol oxidase.
SIGNIFICANCE AND IMPACT OF THE STUDY
Bacillus subtilis will be very useful for the reduction of cholesterol in many fermented foods and as a source of cholesterol oxidase.
目的
研究枯草芽孢杆菌SFF34对胆固醇的降解作用。
方法与结果
在含有0.2%胆固醇的培养基中研究枯草芽孢杆菌SFF34的胆固醇降解及胆固醇氧化酶产生情况。此外，使用气相色谱检测纯化的胆固醇氧化酶对胆固醇的氧化产物。在胆固醇培养基中培养24小时后，胆固醇氧化酶产量达到最高水平（3.14 U ml(-1)）。在胆固醇培养基中培养60小时后，残余胆固醇含量降至0.98 mg g(-1)。从培养上清液中纯化出两种胆固醇氧化酶，其对胆固醇的反应产物鉴定为4-胆甾烯-3-酮。
结论
枯草芽孢杆菌SFF34可降解胆固醇并产生高水平的胞外胆固醇氧化酶。
研究的意义与影响
枯草芽孢杆菌对于许多发酵食品中胆固醇的降低以及作为胆固醇氧化酶的来源将非常有用。
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