Choi Hye Jin, Shin Donghyun, Shin Minhye, Yun Bohyun, Kang Minkyoung, Yang Hee-Jong, Jeong Do-Youn, Kim Younghoon, Oh Sangnam
Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Korea.
Department of Agricultural Convergence Technology, Jeonbuk National University, Jeonju 54896, Korea.
Foods. 2020 Dec 4;9(12):1805. doi: 10.3390/foods9121805.
Many fermented foods are known to have beneficial effects on human and animal health, offering anti-aging and immunomodulatory benefits to host. Microorganisms contained in the fermented foods are known to provide metabolic products possibly improving host health. However, despite of a number of studies on the functional effects of the fermented foods, isolation and identification of the effective bacterial strains in the products are still in progress. The objective of this study was to isolate candidate functional strains in various Korean traditional fermented foods, including ganjang, gochujang, doenjang, and jeotgal, and evaluate their beneficial effects on the host, using as a surrogate animal model. Among the 30 strains isolated, five spp. were selected that increased the expression level of , an innate immune gene of . These strains extended the nematode lifespan and showed intestinal adhesion to the host. Based on the bioinformatic analyses of whole genome sequences and pangenomes, the five strains of were genetically different from the strains found in East Asian countries and previously reported strains isolated from Korean fermented foods. Our findings suggest that the newly isolated strains can be a good candidate for probiotic with further in-depth investigation on health benefits and safety.
许多发酵食品已知对人类和动物健康有益，为宿主提供抗衰老和免疫调节益处。已知发酵食品中含有的微生物会产生可能改善宿主健康的代谢产物。然而，尽管对发酵食品的功能作用进行了大量研究，但产品中有效细菌菌株的分离和鉴定仍在进行中。本研究的目的是从各种韩国传统发酵食品中分离候选功能菌株，包括酱油、辣椒酱、豆瓣酱和腌鱼，并以秀丽隐杆线虫作为替代动物模型评估它们对宿主的有益作用。在分离出的30株菌株中，选择了5株嗜盐放线菌，它们提高了秀丽隐杆线虫先天免疫基因lys-7的表达水平。这些菌株延长了线虫的寿命，并显示出对宿主肠道的黏附性。基于全基因组序列和泛基因组的生物信息学分析，这5株嗜盐放线菌在基因上与东亚国家发现的菌株以及先前报道的从韩国发酵食品中分离出的菌株不同。我们的研究结果表明，新分离的嗜盐放线菌菌株在对健康益处和安全性进行进一步深入研究后，可能是一种很好的益生菌候选菌株。
