Asan-Ozusaglam Meltem, Gunyakti Ayse
Department of Biotechnology and Molecular Biology, Faculty of Science and Letters, Aksaray University, 68100 Aksaray, Turkey.
Food Sci Biotechnol. 2018 Oct 16;28(2):501-509. doi: 10.1007/s10068-018-0494-y. eCollection 2019 Apr.
The study aimed to identify two lactic acid bacteria from human breast milk and evaluate for their probiotic potential. strains showed various degree of antibiotic susceptibility profile to clinical commercial antibiotics. The MA-7 and MA-8 strains have never lost their vitality at pH 2 and 3. MA-8 exhibited more resistance to 0.3 and 1% bile concentrations than MA-7 strain. Both of the strains can tolerate the simulated gastric and intestinal juices. The strains showed high auto-aggregation percentages varied from 95 to 98%. Both isolates also exhibited gamma hemolytic activity. The cholesterol lowering activity of MA-7 and MA-8 strains was varied from 34.84 to 91.15%. The supernatants showed higher anti-cholesterol activity compared to pellets. The results demonstrated that both strains maybe good probiotic candidates and may have potential as health biotherapeutic with cholesterol-lowering effects.
该研究旨在从人母乳中鉴定出两种乳酸菌，并评估它们的益生菌潜力。这些菌株对临床商用抗生素表现出不同程度的抗生素敏感性。MA - 7和MA - 8菌株在pH值为2和3时从未失去活力。MA - 8比MA - 7菌株对0.3%和1%的胆汁浓度表现出更强的抗性。这两种菌株都能耐受模拟胃液和肠液。这些菌株显示出95%至98%的高自聚集百分比。两种分离株也都表现出γ溶血活性。MA - 7和MA - 8菌株的降胆固醇活性在34.84%至91.15%之间。与菌泥相比，上清液显示出更高的抗胆固醇活性。结果表明，这两种菌株可能是良好的益生菌候选菌株，并且可能具有作为具有降胆固醇作用的健康生物治疗剂的潜力。
