Faraj B A, Israili Z H, Kight N E, Smissman E E, Pazdernik T J
J Med Chem. 1976 Jan;19(1):20-5. doi: 10.1021/jm00223a005.
The specificity of an antibody directed against d-(S)-methamphetamine (MA) was determined by competitive binding assay with more than 50 compounds-metabolites, homologs, and analogs of amphetamine. The antibody appears to be specific both for the side chain and the aromatic ring of d-(S)-amphetamine (A). The basic requirements for a compound to be bound to the antibody are (a) an aromatic ring, (b) a basic nitrogen, and (c) a two-carbon chain between the aromatic ring and the nitrogen. A transoid conformation for the phenethylamine skeleton is preferred. The interaction of the antibody with compounds differing from MA or A in side-chain substitutions was directly proportional to the closeness of their structure to MA and/or A. The antibody exhibited greatly reduced affinity for ring-substituted analogs of A; the p-hydroxy metabolite of A did not bind to the antibody. A radioimmunoassay of A is described; it was utilized to study the disposition of A in dogs.
通过与50多种化合物（安非他命的代谢物、同系物和类似物）进行竞争性结合试验，确定了一种针对d-(S)-甲基苯丙胺（MA）的抗体的特异性。该抗体似乎对d-(S)-苯丙胺（A）的侧链和芳香环都具有特异性。化合物与该抗体结合的基本要求是：（a）一个芳香环；（b）一个碱性氮原子；（c）芳香环与氮原子之间有一个双碳链。苯乙胺骨架的反式构象是优选的。该抗体与在侧链取代上不同于MA或A的化合物之间的相互作用与其结构与MA和/或A的接近程度成正比。该抗体对A的环取代类似物的亲和力大大降低；A的对羟基代谢物不与该抗体结合。描述了一种A的放射免疫测定法；它被用于研究A在犬体内的处置情况。
