Jakubović A, McGeer P L
J Toxicol Environ Health. 1976 Jan;1(3):441-7. doi: 10.1080/15287397609529343.
Delta9-Tetrahydrocannabinol (THC) and/or ethanol were administered into the yolk sac of chicken eggs at various times of incubation. Repetitive injections of 1 or 2 mg THC and/or 10 mul of alcohol were fiven. With a schedule of injections on days 2, 4, 7, and 9 with sacrifice on day 11, or on days 2, 4, 7, 9, and 11 with sacrifice on day 15, the body weight was reduced and the liver weight increased. The latter schedule also decreased the weight of the forebrain. Injections of labeled leucine and uridine were also given 1 hr before sacrifice to determine the rate of incorporation of these precursors into protein and RNA. Apparent changes were found in the specific activities of protein and RNA in different parts of the brain and liver. No overall metabolic trends emerged, however. No teratogenic effects were visible with the dosage schedules used.
在孵化的不同时间，将Δ9-四氢大麻酚（THC）和/或乙醇注入鸡蛋的卵黄囊中。重复注射1或2毫克THC和/或10微升酒精。按照在第2、4、7和9天注射并在第11天处死，或者在第2、4、7、9和11天注射并在第15天处死的方案，发现体重减轻而肝脏重量增加。后一种方案还降低了前脑的重量。在处死前1小时也注射了标记的亮氨酸和尿苷，以确定这些前体掺入蛋白质和RNA的速率。在脑和肝的不同部位，蛋白质和RNA的比活性出现了明显变化。然而，没有出现整体代谢趋势。在所使用的剂量方案下，未观察到致畸作用。
