NAADP可从海胆卵中的储备颗粒（即溶酶体相关细胞器）中动员钙离子。
NAADP mobilizes Ca(2+) from reserve granules, lysosome-related organelles, in sea urchin eggs.
作者信息
Churchill Grant C, Okada Yuhei, Thomas Justyn M, Genazzani Armando A, Patel Sandip, Galione Antony
机构信息
Department of Pharmacology, University of Oxford, Mansfield Road, OX1 3QT, Oxford, United Kingdom.
出版信息
Cell. 2002 Nov 27;111(5):703-8. doi: 10.1016/s0092-8674(02)01082-6.
Nicotinic acid adenine dinucleotide phosphate (NAADP) mobilizes Ca(2+) in many cells and species. Unlike other Ca(2+)-mobilizing messengers, NAADP mobilizes Ca(2+) from an unknown store that is not the endoplasmic reticulum, the store traditionally associated with messenger-mediated Ca(2+) signaling. Here, we demonstrate the presence of a Ca(2+) store in sea urchin eggs mobilized by NAADP that is dependent on a proton gradient maintained by an ATP-dependent vacuolar-type proton pump. Moreover, we provide pharmacological and biochemical evidence that this Ca(2+) store is the reserve granule, the functional equivalent of a lysosome in the sea urchin egg. These findings represent an unsuspected mechanism for messenger-mediated Ca(2+) release from lysosome-related organelles.
烟酰胺腺嘌呤二核苷酸磷酸（NAADP）在许多细胞和物种中可动员钙离子（Ca(2+)）。与其他钙离子动员信使不同，NAADP从一个未知的储存库中动员钙离子，该储存库并非内质网，而内质网是传统上与信使介导的钙离子信号传导相关的储存库。在此，我们证明海胆卵中存在一种由NAADP动员的钙离子储存库，其依赖于由ATP依赖的液泡型质子泵维持的质子梯度。此外，我们提供了药理学和生物化学证据，表明这种钙离子储存库是储备颗粒，在海胆卵中其功能等同于溶酶体。这些发现代表了一种意想不到的信使介导的钙离子从溶酶体相关细胞器释放的机制。
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