人类“伤害性刺激特异性”眨眼反射记录的优化刺激和记录参数

Optimized stimulation and recording parameters of human 'nociception specific' blink reflex recordings.

作者信息

Katsarava Z, Ellrich J, Diener H C, Kaube H

机构信息

Department of Neurology, University of Essen, Hufelandstrasse 55, 45122 Essen, Germany.

出版信息

Clin Neurophysiol. 2002 Dec;113(12):1932-6. doi: 10.1016/s1388-2457(02)00307-3.

DOI:10.1016/s1388-2457(02)00307-3

PMID:12464330

Abstract

OBJECTIVE

Few non-invasive methods are available for studying trigeminal nociception in humans. The 'nociception specific' blink reflex (nBR) is elicited by a preferential stimulation of trigeminal nociceptive fibers and is highly sensitive to changes in trigeminal nociception. The aim of this study was to establish its optimal stimulation parameters.

METHODS

The inter-individual variability of R2 responses (41 healthy subjects), symmetry of R2 responses after right vs. left sided stimulation (25 subjects), optimal stimulation parameters (3 groups, each 16 subjects) and re-test reliability over time (15 subjects) were studied.

RESULTS

The nBR had a R2 response with a latency of 44.7+/-7.3ms, but no R1 response. The mean difference of R2 latencies (right vs. left sided stimulation) was 0.4+/-2.1(SD)ms, the mean ratio of the R2 response areas (RA) was 1.05+/-0.39 (SD). Thus, lateral differences of onset latencies greater than 4.6ms (0.4+4.2 (2SD)) or >1.83-fold (1.05+0.78 (2SD)) of RA can be assumed as outside of normal range. Long inter-block intervals of 7min minimized the cumulative habituation of R2 RAs to 23%, onset latencies remained constant. Re-test reliability over 4 weeks was high both for latencies (Crohnbach's alpha=0.85) and RAs (alpha=0.94).

CONCLUSIONS

The nBR with the established stimulation parameters could be used for studying trigeminal nociception.

摘要

目的

在人类中，用于研究三叉神经痛觉的非侵入性方法很少。“痛觉特异性”眨眼反射（nBR）由三叉神经痛觉纤维的优先刺激引发，并且对三叉神经痛觉的变化高度敏感。本研究的目的是确定其最佳刺激参数。

方法

研究了R2反应的个体间变异性（41名健康受试者）、右侧与左侧刺激后R2反应的对称性（25名受试者）、最佳刺激参数（3组，每组16名受试者）以及随时间的重测可靠性（15名受试者）。

结果

nBR有潜伏期为44.7±7.3毫秒的R2反应，但无R1反应。R2潜伏期（右侧与左侧刺激）的平均差异为0.4±2.1（标准差）毫秒，R2反应面积（RA）的平均比率为1.05±0.39（标准差）。因此，可以认为起始潜伏期的侧向差异大于4.6毫秒（0.4 + 4.2（2标准差））或RA的>1.83倍（1.05 + 0.78（2标准差））超出正常范围。7分钟的长组间间隔将R2 RA的累积习惯化最小化至23%，起始潜伏期保持恒定。4周内的重测可靠性对于潜伏期（Cronbach's alpha = 0.85）和RA（alpha = 0.94）都很高。