Savage John R K
34 City Road, Tilehurst, Reading RG31 5HB, UK.
Mutat Res. 2002 Dec;512(2-3):93-109. doi: 10.1016/s1383-5742(02)00066-2.
The application of FISH chromosome painting techniques, especially the recent mFISH (and its equivalents) where all 23 human chromosome pairs can be distinguished, has demonstrated that many chromosome-type structural exchanges are much more complicated (involving more "break-rejoins" and arms) than has hitherto been assumed. It is clear that we have been greatly under-estimating the damage produced in chromatin by such agents as ionising radiation. This article gives a brief historical summary of observations leading up to this conclusion, and after outlining some of the problems surrounding the formation of complex chromosomes exchanges, speculates about possible solutions currently being proposed.
荧光原位杂交（FISH）染色体涂染技术的应用，尤其是最近的多色荧光原位杂交（mFISH，以及与之等效的技术），能够区分所有23对人类染色体，这表明许多染色体类型的结构交换比以往所认为的要复杂得多（涉及更多的“断裂-重接”和染色体臂）。显然，我们一直大大低估了诸如电离辐射等因素对染色质造成的损伤。本文简要回顾了得出这一结论的观察结果的历史概况，并在概述了围绕复杂染色体交换形成的一些问题之后，推测了目前正在提出的可能解决方案。
