He Guanglong, Evalappan Sathesh P, Hirata Hiroshi, Deng Yuanmu, Petryakov Sergey, Kuppusamy Periannan, Zweier Jay L
Center for Biomedical EPR Spectroscopy and Imaging, Davis Heart and Lung Research Institute, The Ohio State University, College of Medicine, Columbus, Ohio 43210-1252, USA.
Magn Reson Med. 2002 Dec;48(6):1057-62. doi: 10.1002/mrm.10302.
Surface coil resonators have been widely used to perform topical EPR spectroscopy. They are usually positioned adjacent to or implanted within the body. For EPR applications these resonators have a number of important advantages over other resonator designs due to their ease of sample accessibility, mechanical fabrication, implementation of electronic tuning and coupling functions, and low susceptibility to sample motions. However, a disadvantage is their B(1) field inhomogeneity, which limits their usefulness for 3D imaging applications. We show that this problem can be addressed by mapping and correcting the B(1) field distribution. We report the use of EPR imaging (EPRI) to map the B(1) distribution of a surface coil resonator. We show that EPRI provides a fast, accurate, and reliable technique to evaluate the B(1) distribution. 3D EPRI was performed on phantoms, prepared using three different saline concentrations, to obtain the B(1) distribution. The information obtained from the phantoms was used to correct the images of living animals. With the use of this B(1) correction technique, surface coil resonators can be applied to perform 3D mapping of the distribution of free radicals in biological samples and living systems.
表面线圈谐振器已被广泛用于进行局部电子顺磁共振光谱学研究。它们通常放置在身体附近或植入体内。对于电子顺磁共振应用，这些谐振器相较于其他谐振器设计具有许多重要优势，这得益于其易于接近样品、便于机械制造、易于实现电子调谐和耦合功能以及对样品运动的低敏感性。然而，一个缺点是其B(1)场不均匀，这限制了它们在三维成像应用中的实用性。我们表明，通过绘制和校正B(1)场分布可以解决这个问题。我们报告了使用电子顺磁共振成像（EPRI）来绘制表面线圈谐振器的B(1)分布。我们表明，EPRI提供了一种快速、准确且可靠的技术来评估B(1)分布。使用三种不同盐水浓度制备的体模进行了三维EPRI，以获得B(1)分布。从体模获得的信息用于校正活体动物的图像。通过使用这种B(1)校正技术，表面线圈谐振器可用于对生物样品和活体系统中自由基的分布进行三维测绘。
