Chafin David
, 1910 East Innovation PARK Drive, Oro Valley, AZ, 85755, USA,
Recent Results Cancer Res. 2015;199:107-17. doi: 10.1007/978-3-319-13957-9_11.
Modern pathology is built around the principle of preserving tissues such that the in vivo molecular status is maintained at levels representative of the disease state. Tissues are immersed in a solution of fixative which slowly inactivates biological activities, thus preserving the sample. Further processing ultimately allows the tissue to be embedded into wax for thin sectioning and staining for interpretation microscopically. Every year, around 7 billion tissue samples are submitted for processing in the United States alone. With this huge workload, histology laboratories are looking for faster methods of performing fixation, which currently require from several hours to days to complete. Ideally, this procedure could be standardized and would be quicker with better preservation over a wide range of biologically relevant molecules.
现代病理学围绕组织保存原则构建，以使体内分子状态维持在代表疾病状态的水平。组织浸入固定剂溶液中，固定剂会缓慢使生物活性失活，从而保存样本。进一步处理最终可使组织嵌入蜡块中进行薄切片和染色，以便显微镜观察解读。仅在美国，每年就有大约70亿个组织样本提交处理。面对如此巨大的工作量，组织学实验室正在寻找更快的固定方法，目前的固定方法需要数小时至数天才能完成。理想情况下，该程序可以标准化，并且在广泛的生物相关分子上能更快且保存得更好。
